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A translational view of cells' secretome analysis - from untargeted proteomics to potential circulating biomarkers
Biochimie ( IF 3.9 ) Pub Date : 2018-05-18 , DOI: 10.1016/j.biochi.2018.05.007
Sandra I. Anjo , Bruno Manadas

The identification of circulating biomarkers capable of being used as routine for diagnosis, prognosis, risk stratification and therapeutic monitoring is still the major aim at clinical proteomics. However, the direct search in blood samples or other clinical relevant biofluids is frequently associated with technical limitations that make the biomarker discovery process extremely difficult when using these types of samples. In this sense, the use of different approaches, such as the analysis of cells' secretomes, can be an important and promising complementary method for the identification and pre-selection of potential circulating biomarkers.

Therefore, the present review is focused on published works exclusively devoted to the use of cells' secretomes for the identification of new circulating biomarkers. Considering these publications, the translational workflow used to identify potential protein circulating biomarkers from untargeted cells' secretome screenings is presented and discussed in this review. This translational workflow follows a 3-phase pipeline (discovery, verification and validation phase) similar to the “triangular strategy” already applied in biomarker discovery using blood or other clinical samples. For each phase it will be indicated the type of samples, the techniques used to analyze the samples, as well as, the major outcomes and methods used to analyze the data obtained.

The examples presented in this review points out the potential of this secretome-based translational pipeline for the identification of new circulating biomarker candidates, with some of the biomarkers pointed out presenting better discriminatory capacity or at least improving the discriminatory capacity of well-established biomarkers. Finally, some of the potential improvements are also identified and discussed in this review, which can increase the visibility and importance of this cells' secretome-based pipeline for the identification of new potential circulating biomarkers.



中文翻译:

细胞分泌组分析的转换视图-从非靶向蛋白质组学到潜在的循环生物标志物

能够用作诊断,预后,风险分层和治疗监测的常规生物标志物的鉴定仍然是临床蛋白质组学的主要目标。然而,在血液样本或其他临床相关生物流体中的直接搜索经常与技术限制相关联,这使得在使用这些类型的样本时生物标志物的发现过程极为困难。从这个意义上讲,使用不同的方法(例如分析细胞的分泌组)可能是一种重要且有前途的补充方法,用于鉴定和预选潜在的循环生物标志物。

因此,本综述着重于已发表的著作,这些著作专门致力于使用细胞的分泌组来鉴定新的循环生物标志物。考虑到这些出版物,本综述介绍并讨论了用于从未靶向细胞的分泌组筛选中识别潜在的蛋白质循环生物标志物的翻译工作流程。该翻译工作流程遵循三个阶段的流程(发现,验证和确认阶段),类似于在使用血液或其他临床样本进行生物标记发现中已经应用的“三角策略”。对于每个阶段,都将指示样本的类型,用于分析样本的技术以及用于分析获得的数据的主要结果和方法。

这篇综述中的例子指出了这种基于分泌组的翻译管道在鉴定新的循环生物标志物候选物中的潜力,其中一些生物标志物指出了更好的鉴别能力或至少提高了公认的生物标志物的鉴别能力。最后,在这篇综述中还鉴定并讨论了一些潜在的改进,这可以提高该细胞基于分泌组的管道对新的潜在循环生物标志物的识别的可见性和重要性。

更新日期:2018-05-18
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