Objective

Collagen fibrils aid in anchoring resin composite restorations to the dentine substrate. The aim of the study was to investigate effect of non-enzymatic glycation on bond strength and durability of demineralized dentine specimens in a modified two-step etch-and-rinse dentine adhesive.

Methods

Dentine surfaces were etched with 37% phosphoric acid, bonded with respective in vitro ethanol and acetone adhesives modified with (m/m, 0, 1%, 2% and 3% ribose), restored with restorative composite–resin, and sectioned into resin–dentine slabs and beams to be stored for 24 h or 12 months in artificial saliva. Bond-strength testing was performed with bond failure analysis. Pentosidine assay was performed on demineralized ribose modified dentine specimens with HPLC sensitive fluorescent detection. The structural variations of ribose-modified dentine were analysed using TEM and human dental pulpal cells were used for cell viability. Three-point bending test of ribose-modified dentine beams were performed and depth of penetration of adhesives evaluated with micro-Raman spectroscopy. The MMP-2 and cathepsin K activities in ribose-treated dentine powder were also quantified using ELISA. Bond strength data was expressed using two-way ANOVA followed by Tukey’s test. Paired T tests were used to analyse the specimens for pentosidine crosslinks. The modulus of elasticity and dentinal MMP-2 and cathepsin K concentrations was separately analyzed using one-way ANOVA.

Results

The incorporation of RB in the experimental two-step etch-and-rinse adhesive at 1% improved the adhesive bond strength without adversely affecting the degree of polymerisation. The newly developed adhesive increases the resistance of dentine collagen to degradation by inhibiting endogenous matrix metalloproteinases and cysteine cathepsins. The application of RB to acid-etched dentine helps maintain the mechanical properties.

Significance

The incorporation of 1%RB can be considered as a potential candidate stabilizing resin dentine bond.

中文翻译：

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核糖修饰的两步蚀刻和冲洗牙本质粘合剂的体外评估

客观的

胶原蛋白原纤维有助于将树脂复合物修复体锚定在牙本质基质上。这项研究的目的是研究非酶糖基化对改良的两步腐蚀和冲洗型牙本质粘合剂中脱盐的牙本质标本的结合强度和持久性的影响。

方法

用37％的磷酸蚀刻牙本质表面，并分别用（m / m，0％，1％，2％和3％核糖），用修复性复合树脂修复，然后切成树脂-牙本质板和梁，在人造唾液中保存24小时或12个月。结合强度测试是通过结合破坏分析进行的。在脱矿核糖修饰的牙本质标本上进行戊糖苷测定，并采用HPLC敏感的荧光检测。使用TEM分析核糖修饰的牙本质的结构变异，并将人类牙髓细胞用于细胞活力。进行了核糖修饰的牙本质束的三点弯曲测试，并通过显微拉曼光谱法评估了粘合剂的渗透深度。核糖处理过的牙本质粉中的MMP-2和组织蛋白酶K活性也使用ELISA进行了定量。使用双向方差分析（ANOVA），然后进行Tukey检验来表示粘结强度数据。配对的T检验用于分析样品中的戊糖苷交联。使用单向方差分析分别分析弹性模量和牙本质MMP-2和组织蛋白酶K的浓度。

结果

在实验的两步蚀刻和漂洗粘合剂中加入RB的比例为1％，可以改善粘合剂的粘合强度，而不会不利地影响聚合度。新开发的粘合剂可通过抑制内源性基质金属蛋白酶和半胱氨酸组织蛋白酶来增强牙本质胶原蛋白的抗降解能力。RB在酸蚀牙本质上的应用有助于保持机械性能。

意义

1％RB的掺入可以被认为是稳定树脂牙本质键的潜在候选物。