New methotrexate-acylglycerols and methotrexate-cyclodextrins (α, β and γ-CD) conjugates were obtained via esterification or transesterification reactions. All reactions were catalysed by esterases namely immobilized Lipase from Candida antarctica B and Lipase from Thermomyces lanuginosus. The use of ultrasound to assist the reactions revealed to be a key factor to obtain high conversion yields on both MTX conjugates. Transesterification reactions including long chain triacylglycerols were only successful when ultrasound was applied. In cyclodextrins esterification a higher number of MTX molecules was also linked to cyclodextrins when ultrasound was used. All the conjugates were characterized by MALDI-TOF and NMR spectroscopy.

通过酯化或酯交换反应获得了新的甲氨蝶呤-酰基甘油和甲氨蝶呤-环糊精（α，β和γ-CD）共轭物。所有反应均由酯酶催化，即固定化的南极假丝酵母B型脂肪酶和嗜热丝霉菌的脂肪酶。超声辅助反应的揭示是在两种MTX缀合物上获得高转化率的关键因素。仅当应用超声时，包括长链三酰基甘油在内的酯交换反应才成功。在环糊精酯化中，当使用超声时，更多数量的MTX分子也与环糊精连接。通过MALDI-TOF和NMR光谱对所有缀合物进行表征。