Vitamin B12 is made by only certain prokaryotes yet is required by a number of eukaryotes such as mammals, fish, birds, worms, and Protista, including algae. There is still much to learn about how this nutrient is trafficked across the domains of life. Herein, we describe ways to make a number of different corrin analogs with fluorescent groups attached to the main tetrapyrrole-derived ring. A further range of analogs were also constructed by attaching similar fluorescent groups to the ribose ring of cobalamin, thereby generating a range of complete and incomplete corrinoids to follow uptake in bacteria, worms, and plants. By using these fluorescent derivatives we were able to demonstrate that Mycobacterium tuberculosis is able to acquire both cobyric acid and cobalamin analogs, that Caenorhabditis elegans takes up only the complete corrinoid, and that seedlings of higher plants such as Lepidium sativum are also able to transport B12.

中文翻译：

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构建荧光类似物以跟踪钴胺素（维生素 B12）在细菌、蠕虫和植物中的吸收和分布。

维生素 B12 仅由某些原核生物制造，但许多真核生物如哺乳动物、鱼类、鸟类、蠕虫和原生生物（包括藻类）都需要维生素 B12。关于这种营养素如何在生命的各个领域被贩卖，还有很多需要了解。在这里，我们描述了制造许多不同的corrin 类似物的方法，这些类似物具有连接到主要四吡咯衍生环上的荧光基团。通过将类似的荧光基团连接到钴胺素的核糖环上，还构建了更多类似物，从而产生一系列完整和不完整的类咕啉，以跟踪细菌、蠕虫和植物的吸收。通过使用这些荧光衍生物，我们能够证明结核分枝杆菌能够获得钴酸和钴胺素类似物，而秀丽隐杆线虫仅吸收完整的类咕啉，