Only a few isoflavones have been isolated from plants of the genus Abronia. The biological properties of compounds isolated from Abronia species have not been well established, and their antisepsis effects have not been reported yet. In the present study, a new C-methylcoumarinochromone, was isolated from Abronia nana suspension cultures. Its structure was deduced as 9,11-dihydroxy-10-methylcoumarinochromone (boeravinone Y, 1) by spectroscopic data analysis and verified by chemical synthesis. The potential inhibitory effects of 1 against high mobility group box 1 (HMGB1)-mediated septic responses were investigated. Results showed that 1 effectively inhibited lipopolysaccharide-induced release of HMGB1 and suppressed HMGB1-mediated septic responses, in terms of reduction of hyperpermeability, leukocyte adhesion and migration, and cell adhesion molecule expression. In addition, 1 increased the phagocytic activity of macrophages and exhibited bacterial clearance effects in the peritoneal fluid and blood of mice with cecal ligation and puncture-induced sepsis. Collectively, these results suggested that 1 might have potential therapeutic activity against various severe vascular inflammatory diseases via inhibition of the HMGB1 signaling pathway.

中文翻译：

---

隔离，一个的合成，和防腐效果Ç从隔离-Methylcoumarinochromone Abronia绦虫细胞培养

从Abronia属植物中分离出的异黄酮很少。从阿布氏菌属物种中分离出的化合物的生物学特性尚未得到很好的确定，其防腐作用尚未见报道。在本研究中，从Abronia nana悬浮培养物中分离出一种新的C-甲基香豆素色酮。通过光谱数据分析推导其结构为9,11-二羟基-10-甲基香豆素色酮（boeravinone Y，1），并通过化学合成进行验证。研究了1对高迁移率族1（HMGB1）介导的败血症反应的潜在抑制作用。结果表明1在降低高通透性，白细胞粘附和迁移以及细胞粘附分子表达方面，有效抑制脂多糖诱导的HMGB1释放并抑制HMGB1介导的败血反应。此外，1增加了巨噬细胞的吞噬活性，并在盲肠结扎和穿刺引起的败血症的小鼠腹膜液和血液中表现出细菌清除作用。总体而言，这些结果表明1可能通过抑制HMGB1信号通路而对各种严重的血管炎性疾病具有潜在的治疗活性。