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Assignment by Negative-Ion Electrospray Tandem Mass Spectrometry of the Tetrasaccharide Backbones of Monosialylated Glycans Released from Bovine Brain Gangliosides
Journal of the American Society for Mass Spectrometry ( IF 3.2 ) Pub Date : 2018-05-11 , DOI: 10.1007/s13361-018-1944-8
Wengang Chai 1 , Yibing Zhang 1 , Laura Mauri 2 , Maria G. Ciampa 2 , Barbara Mulloy 1 , Sandro Sonnino 2 , Ten Feizi 1
Affiliation  

Gangliosides, as plasma membrane-associated sialylated glycolipids, are antigenic structures and they serve as ligands for adhesion proteins of pathogens, for toxins of bacteria, and for endogenous proteins of the host. The detectability by carbohydrate-binding proteins of glycan antigens and ligands on glycolipids can be influenced by the differing lipid moieties. To investigate glycan sequences of gangliosides as recognition structures, we have underway a program of work to develop a “gangliome” microarray consisting of isolated natural gangliosides and neoglycolipids (NGLs) derived from glycans released from them, and each linked to the same lipid molecule for arraying and comparative microarray binding analyses. Here, in the first phase of our studies, we describe a strategy for high-sensitivity assignment of the tetrasaccharide backbones and application to identification of eight of monosialylated glycans released from bovine brain gangliosides. This approach is based on negative-ion electrospray mass spectrometry with collision-induced dissociation (ESI-CID-MS/MS) of the desialylated glycans. Using this strategy, we have the data on backbone regions of four minor components among the monosialo-ganglioside-derived glycans; these are of the ganglio-, lacto-, and neolacto-series.

中文翻译:

负离子电喷雾串联质谱法从牛脑神经节苷脂释放的单唾液酸化聚糖的四糖骨架分配。

神经节苷脂是与血浆膜相关的唾液酸化糖脂,是一种抗原结构,可作为病原体粘附蛋白,细菌毒素和宿主内源蛋白的配体。糖抗原上糖基聚糖抗原和配体的碳水化合物结合蛋白的可检测性可能受不同脂质部分的影响。为了研究神经节苷脂的糖基序列作为识别结构,我们正在进行一项工作计划,以开发由分离的天然神经节苷脂和新糖脂(NGL)组成的“神经节”微阵列,这些糖脂是从糖脂中释放出来的,各自与相同的脂质分子相连。阵列和比较性微阵列结合分析。在这里,在我们研究的第一阶段,我们描述了一种高灵敏度的四糖骨架分配策略,并应用于鉴定从牛脑神经节苷脂释放的八种单唾液酸化聚糖。该方法基于负离子电喷雾质谱,该质谱具有脱唾液酸化聚糖的碰撞诱导解离(ESI-CID-MS / MS)。使用这种策略,我们获得了单唾液酸神经节苷脂衍生的聚糖中四个次要成分的骨架区域的数据。这些是 我们获得了单唾液酸神经节苷脂衍生的聚糖中四个次要成分的骨架区域的数据;这些是 我们获得了单唾液酸神经节苷脂衍生的聚糖中四个次要成分的骨架区域的数据;这些是ganglio - ,-和neolacto -series。
更新日期:2018-05-12
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