当前位置: X-MOL 学术J. Chem. Educ. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Determining the Deacetylation Degree of Chitosan: Opportunities To Learn Instrumental Techniques
Journal of Chemical Education ( IF 3 ) Pub Date : 2018-05-10 00:00:00 , DOI: 10.1021/acs.jchemed.7b00902
Leyre Pérez-Álvarez 1 , Leire Ruiz-Rubio 1 , Jose Luis Vilas-Vilela 1
Affiliation  

To enhance critical thinking and problem-solving skills, a project-based learning (PBL) approach for “Instrumental Techniques” courses in undergraduate physical chemistry was specifically developed for a pharmacy bachelor degree program. The starting point of this PBL was an open-ended question that is close to the student scientist’s interest and related to this lab: “How can we determine the deacetylation degree of chitosan?” Chitosan is a polysaccharide used in a broad range of pharmacy applications because of its unique properties. Many of these properties derive from the presence of primary amino groups (−NH2) in its structure. These −NH2 groups are usually formed by the deacetylation of chitin. For this reason, a proper quantification of the deacetylation degree (DD) of chitosan is important to know whether it can be used in a particular application. This driving question requires an experimental procedure that can be carried out by a wide range of instrumental techniques. In this project, techniques such as conductometry, potentiometry, and 1H NMR were addressed to present an easily reproducible experience for first-year undergraduate students. Students evaluated the applicability of the available instrumental techniques, designed their own laboratory procedures, obtained experimental results, and analyzed data in a challenging experience in which high order thinking and knowledge transfer skills were endorsed. The project described here promotes students’ knowledge of instrumental techniques, and offers them an appropriate scenario to develop transferable skills, such as team-working, problem-solving, and written communication, among others.

中文翻译:

确定壳聚糖的脱乙酰度:学习仪器技术的机会

为了提高批判性思维和解决问题的能力,专门为药房学士学位课程开发了基于项目的学习(PBL)的本科物理化学“仪器技术”课程的方法。该PBL的出发点是一个与学生科学家的兴趣紧密相关且与本实验室相关的开放性问题:“我们如何确定壳聚糖的脱乙酰度?” 壳聚糖是一种多糖,由于其独特的性能而被广泛用于制药领域。这些特性中的许多特性都源于其结构中存在伯氨基(-NH 2)。这些-NH 2基团通常是通过几丁质的脱乙酰作用形成的。因此,壳聚糖脱乙酰度(DD)的正确定量对于了解其是否可用于特定应用非常重要。这个驱动问题需要一个实验程序,可以通过多种仪器技术来执行。在这个专案中,电导法,电位法和1等技术H NMR旨在为一年级本科生提供易于再现的体验。学生评估了可用仪器技术的适用性,设计了自己的实验室程序,获得了实验结果,并在具有挑战性的经验中分析了数据,在这些经验中,高阶思维和知识转移技能得到了认可。此处描述的项目旨在增进学生对乐器技术的了解,并为他们提供适当的场景来发展可转移的技能,例如团队合作,解决问题和书面沟通等。
更新日期:2018-05-10
down
wechat
bug