This laboratory experiment employs an innovative approach to introduce undergraduates to liquid chromatography–tandem mass spectrometry (LC–MS/MS) by developing a method based on multiple reaction monitoring (MRM). During this qualitative investigation, students operated a triple quadrupole mass spectrometer in the MS scan and product ion scan modes to determine precursor and product ions, respectively, for a vanillin, ethyl vanillin, and coumarin standard mixture. After identifying precursor → product ion transitions, students conducted a 2 min liquid chromatography run in MRM mode. Students were astonished by the speed, selectivity, and sensitivity of the LC–MS/MS method compared to that of a liquid chromatograph with ultraviolet detection.

中文翻译：

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通过多反应监测（MRM）方法开发来教学生LC-MS / MS理论和操作

该实验室实验采用一种创新方法，通过开发基于多反应监测（MRM）的方法，向大学生介绍液相色谱-串联质谱（LC-MS / MS）。在此定性研究中，学生以MS扫描和产物离子扫描模式操作了一个三重四极杆质谱仪，分别确定了香兰素，乙基香兰素和香豆素标准混合物的前体离子和产物离子。在确定前体→产物离子跃迁后，学生进行了2分钟的液相色谱，以MRM模式运行。与采用紫外检测的液相色谱仪相比，LC-MS / MS方法的速度，选择性和灵敏度令学生惊讶。