当前位置: X-MOL 学术Aquat. Toxicol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Chlorophyll a fluorescence and transcriptome reveal the toxicological effects of bisphenol A on an invasive cyanobacterium, Cylindrospermopsis raciborskii
Aquatic Toxicology ( IF 4.5 ) Pub Date : 2018-05-09 , DOI: 10.1016/j.aquatox.2018.05.005
Rong Xiang , Junqiong Shi , Hongbo Zhang , Congcong Dong , Li Liu , JunKe Fu , Xinyu He , Yanjun Yan , Zhongxing Wu

Bisphenol A has attracted worldwide attention due to its harmful effects on humans, animals and plants. In this study, the toxicological effects of BPA on Cylindrospermopsis raciborskii were assessed based on chlorophyll a fluorescence and transcriptome analyses. The results showed that the growth of C. raciborskii was significantly inhibited when BPA exceeded 0.1 mg L−1. A marked rise of phase J was observed at a concentration greater than 0.1 mg L−1, while a K phase appeared at 20 mg L−1. The chlorophyll a fluorescence parameters of RC/CS0, F0, φP0, φE0, and ψ0, underwent a significant decline under all treatments of BPA, whereas a significant increase in both VJ and M0 occurred under all concentrations of BPA. Additionally, ABS/RC and DIo/RC markedly increased at 10 mg L−1 and 20 mg L−1. The transcriptome analysis revealed that the genes of photosynthesis, including psbA, psbB, psbC, psbD, apcA, apcB, cpcA, and cpcB, as well as those of chlorophyll and carotenoid biosynthesis, namely hemN, acsF, chlL, chlN, chlP, crtB, pds, were all down-regulated. Moreover, BPA also inhibited the oxidative phosphorylation, glycolysis/gluconeogenesis, citrate cycle (TCA cycle), and fatty acid metabolism in C. raciborskii. Taken together, these results suggest BPA can negatively affect the expression of multiple genes and the vital energy metabolism process to arrest the growth and photosynthesis of C. raciborskii.



中文翻译:

叶绿素荧光和转录组揭示了双酚A对入侵性蓝细菌Cylindrospermopsis raciborskii的毒理作用

双酚A由于其对人类,动物和植物的有害作用而引起了全世界的关注。在这项研究中,基于叶绿素荧光和转录组分析,评估了双酚A对拟南芥的毒理作用。结果表明,当双酚A含量超过0.1 mg L -1时,明显抑制了雷氏梭菌的生长。在大于0.1mg L -1的浓度下观察到J相明显升高,而在20mg L -1处出现K相。叶绿素a荧光RC / CS的参数0,F 0,φ P0,φ E0,和ψ 0,在所有BPA处理下均显着下降,而在所有BPA浓度下V J和M 0均显着增加。另外,ABS / RC和DIo / RC在10mg L -1和20mg L -1时显着增加。转录组分析显示,光合作用的基因包括psbApsbBpsbCpsbDapcAapcBcpcAcpcB,以及叶绿素和类胡萝卜素生物合成的基因hemNacsFchlLchlNchlPcrtBpds均被下调。此外,BPA还抑制了雷氏梭菌的氧化磷酸化,糖酵解/糖异生,柠檬酸盐循环(TCA循环)和脂肪酸代谢。综上所述,这些结果表明,双酚A可以负面影响多个基因的表达和重要能量代谢过程,从而阻止雷氏梭菌的生长和光合作用

更新日期:2018-05-09
down
wechat
bug