Abstract Fluorescent water-dispersible conjugated polymeric nanoparticles (WDCPNs) were synthesized for cellular imaging. Sonogashira polycondensations of various combinations of symmetric monomers (Ax + By, x > 2, y ≥ 2) were firstly conducted in confined nanoreactors to produce soluble conjugated polymeric nanoparticles (SCPNs) with narrow size distribution and tunable fluorescences emission. The subsequent click reaction between the terminal alkyne groups in the SCPNs and azide terminated PEG1000 endowing good dispersity of the WDCPNs in aqueous solutions and stable fluorescence emission. The WDCPNs exhibited bright fluorescence in cellular imaging and showed good biocompatibility as revealed by cell variability assay, demonstrating them as ideal fluorescent probes for biological imaging and detection.

中文翻译：

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用于细胞成像的水分散性共轭聚合物纳米颗粒的可控合成

摘要 合成了荧光水分散性共轭聚合物纳米粒子 (WDCPNs) 用于细胞成像。对称单体 (Ax + By, x > 2, y ≥ 2) 的各种组合的 Sonogashira 缩聚反应首先在密闭的纳米反应器中进行，以产生具有窄尺寸分布和可调荧光发射的可溶性共轭聚合物纳米粒子 (SCPN)。随后在 SCPNs 中的末端炔基和叠氮化物之间的点击反应终止了 PEG1000，使 WDCPNs 在水溶液中具有良好的分散性和稳定的荧光发射。WDCPNs 在细胞成像中表现出明亮的荧光，并显示出良好的生物相容性，如细胞变异性测定所示，表明它们是生物成像和检测的理想荧光探针。