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Synchrotron- and focal plane array-based Fourier-transform infrared spectroscopy differentiates the basalis and functionalis epithelial endometrial regions and identifies putative stem cell regions of human endometrial glands
Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2018-05-09 , DOI: 10.1007/s00216-018-1111-x
Georgios Theophilou , Camilo L. M. Morais , Diane E. Halliwell , Kássio M. G. Lima , Josephine Drury , Pierre L. Martin-Hirsch , Helen F. Stringfellow , Dharani K. Hapangama , Francis L. Martin

The cyclical process of regeneration of the endometrium suggests that it may contain a cell population that can provide daughter cells with high proliferative potential. These cell lineages are clinically significant as they may represent clonogenic cells that may also be involved in tumourigenesis as well as endometriotic lesion development. To determine whether the putative stem cell location within human uterine tissue can be derived using vibrational spectroscopy techniques, normal endometrial tissue was interrogated by two spectroscopic techniques. Paraffin-embedded uterine tissues containing endometrial glands were sectioned to 10-μm-thick parallel tissue sections and were floated onto BaF2 slides for synchrotron radiation-based Fourier-transform infrared (SR-FTIR) microspectroscopy and globar focal plane array-based FTIR spectroscopy. Different spectral characteristics were identified depending on the location of the glands examined. The resulting infrared spectra were subjected to multivariate analysis to determine associated biophysical differences along the length of longitudinal and crosscut gland sections. Comparison of the epithelial cellular layer of transverse gland sections revealed alterations indicating the presence of putative transient-amplifying-like cells in the basalis and mitotic cells in the functionalis. SR-FTIR microspectroscopy of the base of the endometrial glands identified the location where putative stem cells may reside at the same time pointing towards νsPO2 in DNA and RNA, nucleic acids and amide I and II vibrations as major discriminating factors. This study supports the view that vibration spectroscopy technologies are a powerful adjunct to our understanding of the stem cell biology of endometrial tissue.

中文翻译:

基于同步加速器和焦平面阵列的傅立叶变换红外光谱技术可区分基底膜和功能性上皮内膜区域,并确定人子宫内膜的假定干细胞区域

子宫内膜再生的周期性过程表明,它可能包含可以为子细胞提供高增殖潜能的细胞群。这些细胞谱系具有临床意义,因为它们可能代表克隆细胞,也可能参与肿瘤发生以及子宫内膜异位病变的发展。为了确定是否可以使用振动光谱技术得出人子宫组织内假定的干细胞位置,通过两种光谱技术对正常子宫内膜组织进行了询问。将包含子宫内膜腺的石蜡包埋的子宫组织切成10μm厚的平行组织切片,并漂浮在BaF 2上幻灯片用于基于同步加速器辐射的傅立叶变换红外(SR-FTIR)显微光谱和基于球面焦平面阵列的FTIR光谱。根据检查的腺体的位置确定了不同的光谱特征。对所得的红外光谱进行多变量分析,以确定沿纵向和横切腺段的长度相关的生物物理差异。比较腺体横断面的上皮细胞层,发现其改变表明基底细胞中存在假定的瞬时扩增样细胞,而功能细胞中则存在有丝分裂细胞。子宫内膜腺体的SR-FTIR显微光谱法确定了假定的干细胞可能同时指向v s PO的位置2 在DNA和RNA中,核酸以及酰胺I和II振动是主要的判别因素。这项研究支持以下观点:振动光谱技术是我们对子宫内膜组织干细胞生物学理解的有力辅助。
更新日期:2018-05-09
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