Enzyme catalysis, as a green, efficient process, displays exceptional functionality, adaptivity and sustainability. Multi-enzyme catalysis, which can accomplish the tandem synthesis of valuable materials/chemicals from renewable feedstocks, establishes a bridge between single-enzyme catalysis and whole-cell catalysis. Multi-enzyme catalysis occupies a unique and indispensable position in the realm of biological reactions for energy and environmental applications. Two complementary strategies, i.e., compartmentalization and substrate channeling, have been evolved by living organisms for implementing the complex in vivo multi-enzyme reactions (MERs), which have been applied to construct multi-enzyme catalytic systems (MECSs) with superior catalytic activity and stabilities in practical biocatalysis. This tutorial review aims to present the recent advances and future prospects in this burgeoning research area, stressing the features and applications of the two strategies for constructing MECSs and implementing in vitro MERs. The concluding remarks are presented with a perspective on the construction of MECSs through rational combination of compartmentalization and substrate channeling.

中文翻译：

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生物启发的多酶催化体系的构建†

酶催化是一种绿色，高效的过程，显示出出众的功能，适应性和可持续性。多酶催化可以从可再生原料中完成有价值的材料/化学物质的串联合成，在单酶催化和全细胞催化之间建立了一座桥梁。在能源和环境应用的生物反应领域中，多酶催化占据着独特而必不可少的位置。活生物体已经开发出两种互补的策略，即区室化和底物通道化，以在体内实施该复合物多酶反应（MERs），已被用于构建在实际生物催化中具有优异催化活性和稳定性的多酶催化体系（MECS）。本教程的回顾旨在介绍这个新兴研究领域的最新进展和未来前景，强调构建MECS和实施体外MER的两种策略的特点和应用。通过对隔室化和底物通道的合理组合，以总结性观点对MECS的构造进行了介绍。