A new fluorescent sensor, M201-DPA, based on [5]helicene derivative was utilized as dual-analyte sensor for determination of Cu²⁺ or Zn²⁺ in different media and different emission wavelengths. The sensor could provide selective and bifunctional determination of Cu²⁺ in HEPES buffer containing Triton-X100 and Zn²⁺ in Tris buffer/methanol without interference from each other and other ions. In HEPES buffer, M201-DPA demonstrated the selective ON–OFF fluorescence quenching at 524 nm toward Cu²⁺. On the other hand, in Tris buffer/methanol, M201-DPA showed the selective OFF–ON fluorescence enhancement upon the addition of Zn²⁺, which was specified by the hypsochromic shift at 448 nm. Additionally, M201-DPA showed extremely large Stokes shifts up to ∼150 nm. By controlling the concentration of Zn²⁺ and Cu²⁺ in a living cell, the imaging of a HepG2 cellular system was performed, in which the fluorescence of M201-DPA in the blue channel was decreased upon addition of Cu²⁺ and was enhanced in UV channel upon addition of Zn²⁺. The detection limits of M201-DPA for Cu²⁺ and Zn²⁺ in buffer solutions were 5.6 and 3.8 ppb, respectively. Importantly, the Cu²⁺ and Zn²⁺ detection limits of the developed sensors were significantly lower than permitted Cu²⁺ and Zn²⁺ concentrations in drinking water as established by the U.S. EPA and WHO.

中文翻译：

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基于超大斯托克斯位移的[5] Hel烯衍生物的双分析荧光传感器在缓冲溶液中选择性测定Cu ²⁺或Zn ^2+的方法及其在活细胞中的应用

一种新型的基于[5] ic烯衍生物的荧光传感器M201-DPA被用作双分析物传感器，用于测定不同介质和不同发射波长下的Cu ²⁺或Zn ²⁺。该传感器可以提供选择性和双功能的测定，在含有Triton-X100的HEPES缓冲液中的Cu ²⁺和在Tris缓冲液/甲醇中的Zn ²⁺互不干扰。在HEPES缓冲液中，M201-DPA在524 nm处对Cu ²⁺表现出选择性的ON-OFF荧光猝灭。另一方面，在Tris缓冲液/甲醇中，添加Zn后，M201-DPA表现出选择性的OFF-ON荧光增强²⁺，由448 nm处的变色位移指定。此外，M201-DPA表现出非常大的斯托克斯位移，直至约150 nm。通过控制活细胞中Zn ²⁺和Cu ²⁺的浓度，进行了HepG2细胞系统的成像，其中添加Cu ²⁺时蓝色通道中M201-DPA的荧光降低，并增强了。添加Zn ²⁺后在UV通道中^释放。M201-DPA对缓冲溶液中Cu ²⁺和Zn ²⁺的检出限分别为5.6和3.8 ppb。重要的是，Cu ²⁺和Zn ²⁺研发的传感器的检出限明显低于美国EPA和WHO确定的饮用水中允许的Cu ²⁺和Zn ²⁺浓度。