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Uniform polysaccharide composite microspheres with controllable network by microporous membrane emulsification technique
Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2018-05-07 , DOI: 10.1007/s00216-018-1084-9
Hongwu Zhang , Lan Zhao , Yongdong Huang , Kai Zhu , Qibao Wang , Rui Yang , Zhiguo Su , Guanghui Ma

High resolution has been constantly pursued in both preparative and analytical chromatography. Chromatographic media are a key factor during the entire separation process. Tailor-made chromatographic media have gained more attention because of their adjustable structure appropriate for application. Uniform polysaccharide composite microspheres were prepared with a mixture of agarose and dextran solution by membrane emulsification technique for the first time. Their pore structure was deliberately regulated by adjusting both the polysaccharide composition and the molecular weight of dextran. Compared with pure agarose microspheres, polysaccharide composite microspheres had a higher separation resolution and their separation range was controllable. By increasing agarose concentration and decreasing dextran concentration at the same time during the preparation of composite microspheres, the mean pore size increased first and then decreased later, and also the pore size distribution became narrower. By increasing the molecular weight of dextran, the pores became smaller with a narrower pore size distribution. Microspheres with a composition of 10% agarose/2% dextran T40 or 8% agarose/4% dextran T150 showed a higher separation resolution for proteins within range of low molecular weight. Furthermore, the mechanical strength of this composite microsphere was improved by adjusting its composition. Atomic force microscope (AFM) results showed that pores were distributed evenly on both the surface and the inner part of microspheres, beneficial for the passage of biomolecules. These novel uniform polysaccharide composite microspheres have great potential for high-resolution bioseparation.

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中文翻译:

通过微孔膜乳化技术可控网络的均匀多糖复合微球

在制备色谱和分析色谱中一直追求高分离度。色谱介质是整个分离过程中的关键因素。量身定制的色谱介质因其适用于应用的可调节结构而倍受关注。首次通过膜乳化技术由琼脂糖和葡聚糖溶液的混合物制备均匀的多糖复合微球。通过调节多糖组成和右旋糖酐的分子量来有意地调节它们的孔结构。与纯琼脂糖微球相比,多糖复合微球的分离度更高,分离范围可控。在制备复合微球的过程中,通过同时增加琼脂糖浓度和降低右旋糖酐浓度,平均孔径先增大,然后减小,并且孔径分布变窄。通过增加右旋糖酐的分子量,孔变得更小,孔径分布更窄。组成为10%琼脂糖/ 2%葡聚糖T40或8%琼脂糖/ 4%葡聚糖T150的微球对低分子量范围内的蛋白质显示出更高的分离分辨率。此外,通过调节其组成可以改善该复合微球的机械强度。原子力显微镜(AFM)的结果表明,孔隙在微球的表面和内部均一分布,有利于生物分子的通过。

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更新日期:2018-05-07
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