Abstract A novel nanoparticle (NP) delivery system was designed for encapsulation of bioactive compounds for which quercetin was used as a model. A combination of almond gum (AG) and shellac as biopolymers using the antisolvent method was employed to fabricate the NPs. The influence of several significant preparation factors was investigated. Bare shellac particles showed extreme aggregation when exposed to pH 1.2 (gastric acid conditions), whereas the colloidal system containing 0.7% AG was capable to form NPs with particle size of 197nm with no sign of physio-chemical instability at pH 1.2 and with an encapsulation efficiency (EE) of 78%. Addition of small amounts of polysorbate 80 and Quillaja Saponin led to a drastic decrease in particle size to 90 and 116nm, respectively, with an encapsulation efficiency higher than 98%. The morphology characterization of the NPs with Cryo-SEM revealed that most NPs were spherical with rough surfaces, indicating that AG adsorbed onto the shellac surface as was also reflected by electrokineic measurements. DSC and FTIR analyses indicated that inclusion of quercetin within the NPs coincided with a transition from the crystalline to the amorphous state. To conclude, this study presented the formulation of promising nanocarriers applicable not only in the food but also in the pharmaceutical industry.

中文翻译：

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抗溶剂沉淀法制备槲皮素负载杏仁胶紫胶纳米粒的制备与表征

摘要 设计了一种新型纳米颗粒 (NP) 递送系统，用于包封以槲皮素为模型的生物活性化合物。使用抗溶剂法将杏仁胶（AG）和虫胶作为生物聚合物的组合用于制造纳米颗粒。研究了几个重要的制备因素的影响。当暴露于 pH 1.2（胃酸条件）时，裸虫胶颗粒表现出极度聚集，而含有 0.7% AG 的胶体系统能够形成粒径为 197nm 的纳米颗粒，在 pH 1.2 下没有任何物理化学不稳定迹象，并且具有封装效率 (EE) 为 78%。添加少量聚山梨醇酯 80 和 Quillaja Saponin 导致粒径分别显着减小至 90 和 116nm，包封率高于 98%。使用 Cryo-SEM 对 NPs 的形态表征表明，大多数 NPs 是具有粗糙表面的球形，表明 AG 吸附在虫胶表面上，这也反映在电动测量中。DSC 和 FTIR 分析表明，在 NPs 中包含槲皮素与从结晶态到非晶态的转变一致。总之，这项研究提出了不仅适用于食品而且适用于制药行业的有前景的纳米载体的配方。DSC 和 FTIR 分析表明，在 NPs 中包含槲皮素与从结晶态到非晶态的转变一致。总之，这项研究提出了不仅适用于食品而且适用于制药行业的有前景的纳米载体的配方。DSC 和 FTIR 分析表明，在 NPs 中包含槲皮素与从结晶态到非晶态的转变一致。总之，这项研究提出了不仅适用于食品而且适用于制药行业的有前景的纳米载体的配方。