Abstract Functional apple beverages, including organic apple juices and apple juices with added species, were studied on the content of total concentrations of Ca, Fe, Mg and Mn as well as bioaccessibility of these elements and their chemical fractionation patterns. A new simplified sample preparation procedure was developed and validated for determination of Ca, Fe, Mg and Mn by high resolution-continuum source flame atomic absorption spectrometry (HR-CS FAAS). Bioaccessibility of Ca, Fe, Mg and Mn from analyzed apple beverages was assessed using in vitro simulated gastrointestinal digestion while chemical partitioning of elements species was carried out with the aid of tandem-column solid phase extraction (SPE). It was established that Ca, Mg and Mn are highly bioaccessible from functional apple beverages (>94% of total concentrations), significantly contributing to recommended daily intakes (RDIs) of these elements, i.e. up to 11%, 19% and 21% per 1 L, respectively, for Ca, Mg and Mn.

中文翻译：

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基于 HR-CS FAAS 的方法，用于直接测定功能性苹果饮料中 Ca、Fe、Mg 和 Mn 的总浓度，并通过体外胃肠消化和化学分级评估这些元素的生物可及部分的贡献

摘要 研究了功能性苹果饮料，包括有机苹果汁和添加物种的苹果汁，研究了钙、铁、镁和锰的总浓度含量以及这些元素的生物可及性及其化学分馏模式。开发并验证了一种新的简化样品制备程序，用于通过高分辨率连续源火焰原子吸收光谱法 (HR-CS FAAS) 测定 Ca、Fe、Mg 和 Mn。使用体外模拟胃肠消化评估分析的苹果饮料中 Ca、Fe、Mg 和 Mn 的生物可及性，同时借助串联柱固相萃取 (SPE) 进行元素种类的化学分配。已经确定，Ca、Mg 和 Mn 可从功能性苹果饮料中获得高度生物可及性（> 总浓度的 94%），