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Femtomolar Detection by Nanocoated Fiber Label-Free Biosensors
ACS Sensors ( IF 8.9 ) Pub Date : 2018-05-04 00:00:00 , DOI: 10.1021/acssensors.7b00918
Francesco Chiavaioli 1 , Pablo Zubiate , Ignacio Del Villar , Carlos R. Zamarreño , Ambra Giannetti 1 , Sara Tombelli 1 , Cosimo Trono 1 , Francisco J. Arregui , Ignacio R. Matias , Francesco Baldini 1
Affiliation  

The advent of optical fiber-based biosensors combined with that of nanotechnologies has provided an opportunity for developing in situ, portable, lightweight, versatile, and high-performance optical sensing platforms. We report on the generation of lossy mode resonances by the deposition of nanometer-thick metal oxide films on optical fibers, which makes it possible to measure precisely and accurately the changes in optical properties of the fiber-surrounding medium with very high sensitivity compared to other technology platforms, such as long period gratings or surface plasmon resonances, the gold standard in label-free and real-time biomolecular interaction analysis. This property, combined with the application of specialty structures such as D-shaped fibers, permits enhancing the light–matter interaction. SEM and TEM imaging together with X-EDS tool have been utilized to characterize the two films used, i.e., indium tin oxide and tin dioxide. Moreover, the experimental transmission spectra obtained after the deposition of the nanocoatings have been numerically corroborated by means of wave propagation methods. With the use of a conventional wavelength interrogation system and ad hoc developed microfluidics, the shift of the lossy mode resonance can be reliably recorded in response to very low analyte concentrations. Repeated experiments confirm a big leap in performance thanks to the capability to detect femtomolar concentrations in human serum, improving the detection limit by 3 orders of magnitude when compared with other fiber-based configurations. The biosensor has been regenerated several times by injecting sodium dodecyl sulfate, which proves the capability of sensor to be reused.

中文翻译:

通过纳米涂层的无纤维生物传感器检测飞沫。

基于光纤的生物传感器与纳米技术相结合,为现场开发便携式,轻便,多功能,高性能的光学传感平台提供了机会。我们报告了通过在光纤上沉积纳米级金属氧化物膜而产生的有损模共振,这使得与其他光纤相比,可以以非常高的灵敏度精确,准确地测量光纤环绕介质的光学特性变化。技术平台,例如长周期光栅或表面等离振子共振,是无标记和实时生物分子相互作用分析的金标准。这种特性与特殊结构(例如D形纤维)的应用相结合,可以增强光与物质的相互作用。SEM和TEM成像以及X-EDS工具已被用来表征所用的两种薄膜,即氧化铟锡和二氧化锡。而且,在纳米涂层沉积之后获得的实验透射光谱已经通过波传播方法在数值上得到了证实。通过使用常规的波长查询系统和专门开发的微流体技术,响应于非常低的分析物浓度,可以可靠地记录有损模式共振的位移。重复的实验证实了其性能的飞跃,这归功于它能够检测人血清中飞摩尔浓度的能力,与其他基于纤维的配置相比,将检测限提高了3个数量级。通过注入十二烷基硫酸钠将生物传感器再生几次,
更新日期:2018-05-04
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