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OPTIMIZATION OF COLLAGEN-ELASTIN-LIKE POLYPEPTIDE COMPOSITE TISSUE ENGINEERING SCAFFOLDS USING RESPONSE SURFACE METHODOLOGY
Journal of the Mechanical Behavior of Biomedical Materials ( IF 3.9 ) Pub Date : 2018-05-02
Bhuvaneswari Gurumurthy, Jason A. Griggs, Amol V. Janorkar

The ability of a tissue-engineered scaffold to regenerate functional tissues depends on its mechanical and biochemical properties. Though the commonly used collagen scaffolds have good biochemical properties, they fail due to their poor mechanical and physical properties. We have reinforced the collagen matrix with elastin-like polypeptide (ELP) to improve the mechanical and physical properties and optimized the composite composition using a novel statistical method of response surface methodology (RSM). RSM used a central composite design to correlate the 2 input factor variables (collagen and ELP concentrations) and 3 output objectives (tensile strength, elastic modulus, and toughness) using a second order polynomial equation. Upon uniaxial tensile testing and subsequent RSM optimization, a composite prepared using 6 mg/mL collagen and 18 mg/mL ELP was identified as having an optimal combination of all the three tensile properties. Physical properties of the 6:18 mg/mL composite versus the 6:0 mg/mL collagen-only hydrogel characterized by swelling ratio, differential scanning calorimetry, and FTIR spectroscopy revealed that the addition of ELP reduced the residual water content in the composites and provided evidence of the presence of collagen-ELP interactions. Scanning electron microscopy images of the collagen-only hydrogel showed porous fibrillar and dense afibrillar collagenous microstructure, but the collagen-ELP composite showed a dense collagenous microstructure with characteristic ELP aggregates. We surmise that because of the low water content and dense microstructure, the 6:18 mg/mL collagen-ELP composite had improved mechanical properties. Taken together, the composites prepared in this research can form good quality, rigid porous structures required for tissue engineering applications.



中文翻译:

响应表面法优化胶原弹性蛋白样多肽复合组织工程支架

组织工程支架再生功能组织的能力取决于其机械和生化特性。尽管常用的胶原蛋白支架具有良好的生化特性,但由于其较差的机械和物理性能而失败。我们用弹性蛋白样多肽(ELP)增强了胶原蛋白基质,以改善机械和物理性能,并使用一种新型的响应面统计方法(RSM)来优化复合材料的成分。RSM使用了中央复合设计,使用二阶多项式方程将2个输入因子变量(胶原蛋白和ELP浓度)与3个输出目标(抗张强度,弹性模量和韧性)相关联。经过单轴拉伸测试和随后的RSM优化后,使用6 mg / mL胶原蛋白和18 mg / mL ELP制备的复合材料被确定为具有所有三种拉伸性能的最佳组合。6:18 mg / mL复合材料与6:0 mg / mL仅胶原蛋白的水凝胶的物理特性以溶胀率,差示扫描量热法和FTIR光谱为特征,表明添加ELP可以降低复合材料中残留的水分,提供了存在胶原蛋白-ELP相互作用的证据。仅胶原蛋白水凝胶的扫描电子显微镜图像显示了多孔的原纤维和致密的原纤维胶原微结构,但是胶原蛋白-ELP复合材料显示了具有特征性ELP聚集体的致密胶原微结构。我们推测由于水含量低和微观结构致密,因此6:18 mg / mL胶原蛋白-ELP复合材料具有改善的机械性能。综上所述,本研究中制备的复合材料可形成组织工程应用所需的高质量,刚性的多孔结构。

更新日期:2018-05-03
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