Abstract In the present study, electrospun polyethylene terephthalate nanofibers doped with graphene oxide nanoparticles (GO-PET) was fabricated and used as an effective adsorbent in micro-solid phase extraction (μ-SPE) of tetracycline (TC) and cefotaxime (CF) from honey samples prior to HPLC. The UV–vis spectrophotometer was utilized in the optimization step. The adsorbent was fully characterized using SEM, FT-IR, BET, and BJH methods. The specific surface area and average pore size of the adsorbent were 79.5 m2 g−1 and 4.83 nm, respectively. The honey samples were dissolved in water (5% w/v) at 50 °C for 10 min, centrifuged at 4000 rpm for 15 min and finally filtered using a filter paper before performing the extraction. The important factors of the method were studied to obtain the optimal conditions. Under the optimum conditions (sample solution, 100 mL; adsorbent, 40 mg; pH 5; extraction time, 10 min; desorption solvent; MeOH, 200 μL), the limits of detection for TC and CF were 15.3 and 3.0 μg kg−1. The linear dynamic range (LDR) was obtained as 10–5000 μg kg−1 with the determination coefficients (R2) of 0.9939 and 0.9959, respectively. The real sample extraction recoveries were 89–94% and 95–98% for TC and CF, respectively. The intra-day and inter-day precisions were 5.6 and 4.9% (n = 3) for TC, and 3.6 and 4.5% (n = 9) for CF.

中文翻译：

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电纺纳米纤维微固相萃取-高效液相色谱法测定蜂蜜中四环素和头孢噻肟残留

摘要 在本研究中，制备了掺杂氧化石墨烯纳米粒子 (GO-PET) 的电纺聚对苯二甲酸乙二醇酯纳米纤维，并将其用作四环素 (TC) 和头孢噻肟 (CF) 微固相萃取 (μ-SPE) 的有效吸附剂。 HPLC 之前的蜂蜜样品。在优化步骤中使用了紫外-可见分光光度计。使用 SEM、FT-IR、BET 和 BJH 方法对吸附剂进行了全面表征。吸附剂的比表面积和平均孔径分别为 79.5 m2 g-1 和 4.83 nm。将蜂蜜样品在 50 °C 下溶于水 (5% w/v) 10 分钟，以 4000 rpm 离心 15 分钟，最后用滤纸过滤，然后进行提取。对该方法的重要因素进行了研究，以获得最佳条件。在最佳条件下（样品溶液，100 mL；吸附剂，40 mg；pH 5；提取时间，10 min；解吸溶剂；MeOH，200 μL），TC 和 CF 的检测限分别为 15.3 和 3.0 μg kg-1 . 线性动态范围 (LDR) 为 10–5000 μg kg-1，决定系数 (R2) 分别为 0.9939 和 0.9959。TC 和 CF 的实际样品提取回收率分别为 89-94% 和 95-98%。TC 的日内和日间精度分别为 5.6% 和 4.9% (n = 3)，CF 为 3.6% 和 4.5% (n = 9)。TC 和 CF 的实际样品提取回收率分别为 89-94% 和 95-98%。TC 的日内和日间精度分别为 5.6% 和 4.9% (n = 3)，CF 为 3.6% 和 4.5% (n = 9)。TC 和 CF 的实际样品提取回收率分别为 89-94% 和 95-98%。TC 的日内和日间精度分别为 5.6% 和 4.9% (n = 3)，CF 为 3.6% 和 4.5% (n = 9)。