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Profiling of Turkish propolis subtypes: Comparative evaluation of their phytochemical compositions, antioxidant and antimicrobial activities
LWT - Food Science and Technology ( IF 6 ) Pub Date : 2018-04-30
Petar Ristivojević, Ivica Dimkić, Etil Guzelmeric, Jelena Trifković, Magdalena Knežević, Tanja Berić, Erdem Yesilada, Dušanka Milojković-Opsenica, Slaviša Stanković

Comprehensive analysis of phenolic profiles of botanically different subtypes of Turkish propolis samples were performed using UHPLC–LTQ/Orbitrap/MS/MS method, and additionally total phenolic (TPC) and total flavonoid contents (TFC) as well as their antioxidative activities were evaluated by spectrophotometry. Antimicrobial activity of Turkish propolis against oral cavity bacteria from the genus Streptococcus (S. pyogenes, S. sanguinis, S. mutans) and Candida albicans ATCC 10231 was determined by diffusion and microdilution methods. Extensive fingerprint analysis of Turkish propolis revealed the presence of fifty one phenolic compounds, with fifteen quantified which confirm their affiliation to the two subtypes of the European propolis. All analysed samples have shown antimicrobial potential against all tested bacteria, with S. pyogenes being the most sensitive one. Turkish propolis, especially its orange subtype, can be considered as the high-quality product due to its rich phenolic and flavonoid content, strong antioxidative and antimicrobial activities. Turkish propolis could be, therefore, a good raw material for food and pharmaceutical industry.



中文翻译:

土耳其蜂胶亚型概况分析:对其植物化学成分,抗氧化剂和抗菌活性的比较评估

使用UHPLC–LTQ / Orbitrap / MS / MS方法对土耳其蜂胶植物不同亚型的酚类成分进行了综合分析,此外,还通过以下方法评估了总酚类(TPC)和总类黄酮含量(TFC)以及它们的抗氧化活性。分光光度法。针对从该属口腔细菌土耳其蜂胶的抗微生物活性链球菌化脓性链球菌血链球菌变异链球菌)和白色念珠菌通过扩散和微稀释法确定ATCC 10231。土耳其蜂胶的大量指纹分析表明,存在五十一种酚类化合物,其中十五种被定量,这证实了它们与欧洲蜂胶的两种亚型有关。所有分析的样品均显示出对所有测试细菌的抗菌潜力,化脓性链球菌是最敏感的一种。土耳其蜂胶,尤其是其橙色亚型,由于其丰富的酚和类黄酮含量,强大的抗氧化和抗菌活性,可以被认为是高质量的产品。因此,土耳其蜂胶可能是食品和制药业的良好原料。

更新日期:2018-05-01
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