Samples of chia seeds (Salvia hispánica L.) were degreased by cold pressing and the residue obtained named degreased chia seeds (DCS) had the antioxidants extract obtained. A 2² experimental design was employed in order to evaluate the factors ethanol concentration and extraction time. The optimized conditions were extraction time 15 min with ethanol 70% where the activity (RSA), evaluated by the DPPH (2,2-diphenyl-1-picryl-hydrazyl) method was 94.76%. For this extract, the total phenolics content was 359.64 mg EGA/100 g. A pseudo-univariate calibration model is proposed as an alternative methodology to determine the %RSA by using the relative concentration from multivariate curve resolution with alternating least squares (MCR-ALS) from near-infrared (NIR) spectra of the antioxidant extract from DCS. The results suggest the possibility of %RSA determination directly from NIR spectra from extracts from DCS by using the multivariate resolution method MCR-ALS.

通过冷压对奇亚籽（SalviahispánicaL .）样品进行脱脂，所获得的残留物称为脱脂奇亚籽（DCS）具有获得的抗氧化剂提取物。A 2 ²为了评估乙醇浓度和提取时间的因素，采用了实验设计。优化的条件是用70％乙醇萃取15分钟的时间，其中通过DPPH（2,2-二苯基-1-吡啶基-肼基）方法评估的活性（RSA）为94.76％。对于该提取物，总酚含量为359.64 mg EGA / 100 g。建议使用伪单变量校准模型作为一种替代方法，通过使用来自DCS的抗氧化剂提取物的近红外（NIR）光谱的多变量曲线分辨率和最小二乘（MCR-ALS）的相对浓度来确定％RSA。结果表明，使用多元分辨率方法MCR-ALS可直接从DCS提取物中的NIR光谱中直接测定％RSA。