The use of multiple‐pulse amperometry (MPA) for the determination of narrow therapeutic index (NTI) drugs using batch injection analysis (BIA) with carbon screen‐printed electrodes (SPE) is proposed, seeking to develop a practical and low‐cost analysis kit for application in routine quality control of these drugs. The electrochemical behaviors of aminophylline, carbamazepine, clindamycin, colchicine, minoxidil, prazosin, procainamide, theophylline, warfarin and verapamil were evaluated in different electrolytes, but just one, the 0.1 mol L⁻¹ phosphate buffer, pH 7.0, was chosen for determination of all the analytes. The amperometric detection was optimized as a function of the best oxidation potential for carbon SPE for each analyte, which was in a range from 0.7 to 1.1 V. The injection conditions were determined as a function of the velocity and the volume injected by the BIA system, which were 92.5 μL s⁻¹ and 100 μL, respectively. Under these conditions, a good repeatability (RSD<3 %), high analytical frequency (>215 determinations per hour), large linear ranges and low LOD (<0.42 μmol L⁻¹) for all the NTI drugs were obtained. Furthermore, the proposed method provided an easy qualitative analysis of the investigated analytes using MPA detection. The addition‐recovery studies in pharmaceutical samples containing NTI drugs and the comparison with official methods showed that the proposed analysis Kit is a very fast, simple and efficient alternative for quantification of these analytes.

中文翻译：

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快速简便的电化学分析试剂盒，用于窄治疗指数药物的质量控制

提出了使用多脉冲安培法（MPA）和碳丝网印刷电极（SPE）的批量注射分析（BIA）来测定窄治疗指数（NTI）药物的方法，以寻求开发一种实用且低成本的分析方法试剂盒，用于这些药物的常规质量控制。在不同的电解质中评估了氨茶碱，卡马西平，克林霉素，秋水仙碱，米诺地尔，吡唑嗪，普鲁卡因酰胺，茶碱，华法林和维拉帕米的电化学行为，但仅用一种，即0.1 mol L ^-1选择pH 7.0的磷酸盐缓冲液测定所有分析物。根据每种分析物的碳SPE最佳氧化电位（在0.7至1.1 V范围内）优化安培检测。注入条件根据BIA系统注入的速度和体积确定分别为92.5μLs ^-1和100μL。在这些条件下，良好的重复性（RSD <3％），高分析频率（每小时> 215次测定），大线性范围和低LOD（<0.42μmolL ^-1）），因为所有的NTI药物均已获得。此外，所提出的方法使用MPA检测对所研究的分析物进行了简单的定性分析。对包含NTI药物的药物样品进行加法回收研究以及与官方方法的比较表明，所提出的分析试剂盒是用于定量分析这些分析物的非常快速，简单且有效的替代方法。