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Carbon‐nanotube Modified Screen‐printed Electrode for the Simultaneous Determination of Nitrite and Uric Acid in Biological Fluids Using Batch‐injection Amperometric Detection
Electroanalysis ( IF 3 ) Pub Date : 2018-04-18 , DOI: 10.1002/elan.201800189
Larissa P. Caetano 1 , Ana P. Lima 2 , Thiago F. Tormin 2 , Eduardo M. Richter 2 , Foued S. Espindola 1 , Françoise V. Botelho 1 , Rodrigo A. A. Munoz 2
Affiliation  

A portable electroanalytical system applied for rapid and simultaneous determination of uric acid (UA) and nitrite (NIT) in human biological fluids (urine, saliva and blood) is reported. The system is based on batch‐injection analysis with multiple‐pulse amperometric (BIA‐MPA) detection using screen‐printed electrodes (SPEs) modified with multi‐walled carbon nanotubes. Sample dilution in optimized electrolyte (0.1 mol L−1 Britton‐Robinson buffer pH 2) followed by injection of 100 μL on the electrode surface using an electronic micropipette is performed. UA is detected at +0.45 V and both UA+NIT at +0.70 V. Linear calibration plots for UA and NIT were obtained over the range of 1–500 μmol L−1 with detection limits of 0.05 and 0.06 μmol L−1, respectively. For comparison, a differential‐pulse voltammetric (DPV) method was optimized, and linear calibration plots for UA and NIT were obtained over range of 1–30 μmol L−1 and 1–40 μmol L−1 with detection limits of 0.1 and 0.3 μmol L−1, respectively. BIA‐MPA is highly precise (RSD<1.3 %), fast (160 h−1) and free from sample‐matrix interferences as recovery values ranged from 77 to 121 % for spiked samples (short contact time of sample aliquot with SPE). Contrarily, recovery tests conducted using DPV did not provide adequate recovery values (>150 %), probably due to the longer contact time of the SPE with the biological samples during analysis leading to a severe interference of sample matrices.

中文翻译:

碳纳米管修饰的丝网印刷电极,用于批次注入安培检测法同时测定生物流体中的亚硝酸盐和尿酸

报道了一种便携式电分析系统,该系统用于快速,同时测定人类生物体液(尿,唾液和血液)中的尿酸(UA)和亚硝酸盐(NIT)。该系统基于使用多壁碳纳米管修饰的丝网印刷电极(SPE)的多脉冲安培(BIA-MPA)检测进行批注入分析。在优化的电解质(0.1 mol L -1 Britton-Robinson缓冲液pH 2)中稀释样品,然后使用电子微量移液器在电极表面上注入100μL。在+0.45 V时检测到UA,而在+0.70 V时检测到UA + NIT。获得UA和NIT的线性校正图在1–500μmolL -1的范围内,检出限为0.05和0.06μmolL -1, 分别。为了进行比较,对微分脉冲伏安法(DPV)进行了优化,并获得了UA和NIT的线性校准图,其检出限为0.1和0.3 ,范围为1–30μmolL -1和1–40μmolL -1。分别为μmolL -1。BIA-MPA具有很高的精确度(RSD <1.3%),快速(160 h -1)且不受样品基质干扰,因为加标样品的回收率范围从77%到121%(样品等分试样与SPE的接触时间短)。相反,使用DPV进行的回收率测试不能提供足够的回收率(> 150%),这可能是由于SPE在分析过程中与生物样品的接触时间较长,导致样品基质受到严重干扰。
更新日期:2018-04-18
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