Carbohydrate‐appended TQNPEN [N,N,N′,N′‐tetrakis(2‐quinolylmethyl)‐3‐aza‐1,5‐pentanediamine] derivatives were developed as intracellular fluorescent Cd²⁺ sensors. Glucose (L1), galactose (L2), and maltose (L3) were utilized as sugar moieties. All glycosylated derivatives exhibit a fluorescence increase (I_Cd/I₀ = 11–25) at 415 nm upon addition of 1 equiv. of Cd²⁺; however, 1 equiv. of Zn²⁺ induces negligible fluorescence change due to weak metal–ligand interaction (I_Zn/I₀ = 3, I_Zn/I_Cd = 11–31 %). The properties of the Cd²⁺ complex with parent TQNPEN, including maximum fluorescence wavelength and lifetime, Cd²⁺ specificity of the fluorescence, and strong metal binding affinity, were well preserved in L1–L3. The intracellular Cd²⁺ detection was successfully achieved by glucose‐ and galactose‐appended derivatives L1 and L2, likely due to enhanced cell membrane permeability and intracellular distribution in HeLa cells.

中文翻译：

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用于细胞内Cd2 +荧光检测的碳水化合物附加的TQNPEN [N，N，N'，N'-四（2-喹啉基甲基）-3-氮杂-1,5-戊二胺]衍生物

开发了添加碳水化合物的TQNPEN [ N，N，N'，N'-四（2-喹啉基甲基）-3-氮杂-1,5-戊二胺]衍生物作为细胞内荧光Cd ²⁺传感器。葡萄糖（L1），半乳糖（L2）和麦芽糖（L3）被用作糖部分。加入1当量后，所有糖基化衍生物在415 nm处都显示出荧光增强（I _Cd / I ₀ = 11–25）。镉²⁺ ; 但是，相当于1个当量。由于弱的金属-配体相互作用，Zn ^2+的诱导荧光变化可忽略不计（I _Zn/ I ₀ = 3，I _Zn / I _Cd = 11–31％）。具有母体TQNPEN的Cd ^2+配合物的特性，包括最大的荧光波长和寿命，荧光的Cd ²⁺特异性以及强大的金属结合亲和力，在L1 - L3中得到了很好的保留。葡萄糖和半乳糖附加的衍生物L1和L2成功实现了细胞内Cd ^2+的检测，这可能是由于HeLa细胞中细胞膜通透性增强和细胞内分布所致。