This review discussed about the green biosynthesis of magnetite nanoparticles (Fe₃O₄-NPs) and the biomedical applications, which mainly focus on the targeted anticancer drug delivery. Fe₃O₄-NPs have been studied and proved that Fe₃O₄-NPs can be used in various fields of application, due to “superparamagnetic” property that Fe₃O₄-NPs possessed. In targeted drug delivery system, drug loaded Fe₃O₄-NPs can accumulate at the tumor site by the aid of external magnetic field. This can increase the effectiveness of drug release to the tumor site and vanquish cancer cells without harming healthy cells. In order to apply Fe₃O₄-NPs in human body, Fe₃O₄-NPs have to be biocompatible and biodegradable to minimize the toxicity. So, green biosynthesis plays a crucial role as the biosynthesized Fe₃O₄-NPs is safe to be consumed by human because the materials used are from biological routes, such as plant extract and natural polymer. However, biosynthesis using plant extract is the most popular among them all as plant extract can act as both reducing and stabilizing agents in the synthesizing process of nanoparticles. This approach is not merely simple, yet economic and less waste production, which is environmental friendly. Several biomedical applications of Fe₃O₄-NPs are included in this review, but anticancer drug delivery study is discussed in detail. The criteria for Fe₃O₄-NPs to be used as drug delivery vehicle are discussed so as to study the optimum condition of Fe₃O₄-NPs in drug delivery application. Many researches showed the promising results of Fe₃O₄-NPs in treating cancer cells via in vitro study. Hence, this review is significant which summarize the vital points of Fe₃O₄-NPs in targeted anticancer drug delivery system. Conclusions have been made according to the literature reviewed and some points of view were proposed for future study.

这篇综述讨论了磁铁矿纳米粒子（Fe ₃ O ₄ -NPs）的绿色生物合成及其生物医学应用，这些研究主要集中在靶向抗癌药物的递送上。的Fe ₃ ö ₄ -nps进行了研究，证明了的Fe ₃ ö ₄ -nps可以在各种应用领域中使用，由于“超顺磁性”属性的Fe _3个ö ₄ -nps附体。在靶向药物输送系统中，载有药物的Fe ₃ O ₄-NP可以在外部磁场的帮助下积聚在肿瘤部位。这可以增加药物释放到肿瘤部位并征服癌细胞而不损害健康细胞的有效性。为了将Fe ₃ O ₄ -NP应用于人体，Fe ₃ O ₄ -NP必须具有生物相容性和可生物降解性，以使毒性最小。因此，绿色生物合成在生物合成Fe ₃ O _4中起着至关重要的作用。-NP被人类安全食用，因为所使用的材料来自生物途径，例如植物提取物和天然聚合物。然而，使用植物提取物进行生物合成是最流行的方法，因为植物提取物在纳米颗粒的合成过程中既可以充当还原剂，也可以充当稳定剂。该方法不仅简单，而且经济且浪费少，这是环境友好的。Fe ₃ O ₄ -NPs的几种生物医学应用包括在本综述中，但详细讨论了抗癌药物的递送研究。讨论了Fe ₃ O ₄ -NPs用作药物传递载体的标准，以研究Fe ₃ O ₄的最佳条件-NPs在药物输送中的应用。许多研究通过体外研究表明，Fe ₃ O ₄ -NPs在治疗癌细胞方面有希望的结果。因此，本综述是有意义的，其总结了Fe ₃ O ₄ -NP在靶向抗癌药物递送系统中的要点。根据所回顾的文献得出了结论，并提出了一些观点以供将来研究。