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Temperature responsive nanoparticles: poloxamers as a modulator of Förster resonance energy transfer (FRET)
Nanoscale ( IF 6.7 ) Pub Date : 2018-04-26 00:00:00 , DOI: 10.1039/c8nr01278d Oleksandr Klep 1, 2, 2, 3, 4 , Yuriy Bandera 1, 2, 2, 3, 4 , Stephen H. Foulger 1, 2, 2, 3, 4
Nanoscale ( IF 6.7 ) Pub Date : 2018-04-26 00:00:00 , DOI: 10.1039/c8nr01278d Oleksandr Klep 1, 2, 2, 3, 4 , Yuriy Bandera 1, 2, 2, 3, 4 , Stephen H. Foulger 1, 2, 2, 3, 4
Affiliation
An effective strategy to control the Förster resonance energy transfer (FRET) of a donor/acceptor emitter pair that were attached to a 60 nm poly(propargyl acrylate)(PA) nanoparticle using temperature variations was developed. The size dependent properties of a poly-(ethylene oxide)-poly-(propylene oxide)-poly-(ethylene oxide) (PEO-PPO-PEO) block copolymer (poloxamer) was exploited to vary the spatial separation of the emitters and vary the FRET efficiency. Specifically, a 2% change in FRET efficiency between the donor/acceptor pair was achieved per 1 °C change in temperature from 49 °C to 60 °C when using a poloxamer of 2950 g mol−1 molecular weight, with sections of PPO consisting of 32 repeat units, PEO sections consisting of 12 repeat units and a lower critical solution temperature (LCST) of 58 °C. The methodology presented in this effort is easily extended to other temperature regimes through a judicious choice in poloxamer and corresponding LCST.
中文翻译:
温度响应性纳米颗粒:泊洛沙姆作为Förster共振能量转移(FRET)的调节剂
开发了一种有效的策略来控制温度变化控制的60 nm聚(丙烯酸丙炔丙酯)(PA)纳米粒子附着的供体/受体发射体对的Förster共振能量转移(FRET)。利用聚(环氧乙烷)-聚(环氧丙烷)-聚(环氧乙烷)(PEO-PPO-PEO)嵌段共聚物(泊洛沙姆)的尺寸依赖性特性来改变发射极的空间间隔并改变FRET效率。具体而言,当使用2950 g mol -1的泊洛沙姆时,温度从49°C到60°C每变化1°C,供体/受体对之间的FRET效率就会发生2%的变化。分子量,PPO部分由32个重复单元组成,PEO部分由12个重复单元组成,较低的临界溶液温度(LCST)为58°C。通过谨慎地选择泊洛沙姆和相应的LCST,可以轻松地将这项工作中介绍的方法扩展到其他温度范围。
更新日期:2018-04-26
中文翻译:
温度响应性纳米颗粒:泊洛沙姆作为Förster共振能量转移(FRET)的调节剂
开发了一种有效的策略来控制温度变化控制的60 nm聚(丙烯酸丙炔丙酯)(PA)纳米粒子附着的供体/受体发射体对的Förster共振能量转移(FRET)。利用聚(环氧乙烷)-聚(环氧丙烷)-聚(环氧乙烷)(PEO-PPO-PEO)嵌段共聚物(泊洛沙姆)的尺寸依赖性特性来改变发射极的空间间隔并改变FRET效率。具体而言,当使用2950 g mol -1的泊洛沙姆时,温度从49°C到60°C每变化1°C,供体/受体对之间的FRET效率就会发生2%的变化。分子量,PPO部分由32个重复单元组成,PEO部分由12个重复单元组成,较低的临界溶液温度(LCST)为58°C。通过谨慎地选择泊洛沙姆和相应的LCST,可以轻松地将这项工作中介绍的方法扩展到其他温度范围。