Native mass spectrometry (MS) is an emerging approach for characterizing biomacromolecular structure and interactions under physiologically relevant conditions. In native MS measurement, intact macromolecules or macromolecular complexes are directly ionized from a non-denaturing solvent, and key noncovalent interactions that hold the complexes together can be preserved for MS analysis in the gas phase. This technique provides unique multi-level structural information such as conformational changes, stoichiometry, topology and dynamics, complementing conventional biophysical techniques. Despite the maturation of native MS and greatly expanded range of applications in recent decades, further dissemination is needed to make the community aware of such a technique. In this review, we attempt to provide an overview of the current body of knowledge regarding major aspects of native MS and explain how such technique contributes to the characterization of biomacromolecular higher-order structure and interactions.

天然质谱（MS）是在生理相关条件下表征生物大分子结构和相互作用的新兴方法。在天然MS测量中，完整的大分子或大分子复合物直接从非变性溶剂中电离，并且可以保留将复合物结合在一起的关键非共价相互作用，以便在气相中进行MS分析。该技术提供了独特的多级结构信息，例如构象变化，化学计量，拓扑和动力学，是对传统生物物理技术的补充。尽管近几十年来本地MS已成熟并且应用范围大大扩展，但仍需要进一步传播以使社区意识到这种技术。在这篇评论中，