In this study, a photo-bioelectrochemical fuel cell was constructed for photocurrent generation by illuminating the electrodes within an aqueous solution. In this purpose, gold electrode was coated with poly 4-(4H-Dithieno [3,2-b:2′,3′-d]pyrol-4-yl) aniline, P(DTP-Ph-NH₂) conductive polymer film by using electrochemical polymerization. Then, P(DTP-Ph-NH₂) conductive polymer film coated surface was electrochemically modified with cytochrome C which covalently linked onto the surface via bis-aniline functionality of the polymer film and formed crosslinked-structure. The thylakoid membrane was attached on the surface of this electrode by using bissulfosaxinimidyl suberate (BS₃) and used as photo-anode in photo-bioelectrochemical fuel cell. The photo-cathode of the photo-bioelectrochemical fuel cell fabrication was followed by the modification of conductive polymer poly[5-(4H-dithieno [3,2-b:2′,3′-d]pyrol-4-yl) naphtalene-1-amine] film coating, glutaraldehyde activation, and bilirubin oxidase enzyme immobilization. During the photosynthesis occurring in thylakoid membrane under the light, water was oxidized and separated; while oxygen was released in anode side, the cathode side was reduced the oxygen gas into the water via a bio-electro-catalytic method. The cytochrome C was used for binding of thylakoid membrane to the electrode surface and play an important role for transferring of electrons released as a result of photosynthesis.

在这项研究中，构建了一种光生化电化学燃料电池，通过照射水溶液中的电极来产生光电流。为此，金电极涂有聚4-（4H-Dithieno [3,2-b：2'，3'-d] pyrol-4-yl）苯胺，P（DTP-Ph-NH ₂）导电聚合物膜通过使用电化学聚合。然后，用细胞色素C对P（DTP-Ph-NH ₂）导电聚合物膜涂覆的表面进行电化学修饰，所述细胞色素C通过聚合物膜的双苯胺官能团共价连接至表面并形成交联结构。使用辛二酸二磺基异辛酯（BS _3）将类囊体膜附着在该电极的表面上。），并用作光生化电化学燃料电池中的光阳极。在光生化电化学燃料电池制造的光阴极之后，对导电聚合物聚[5-（4H-二硫代[3,2-b：2'，3'-d]吡咯-4-基]萘）进行改性-1-胺]薄膜包衣，戊二醛活化和胆红素氧化酶固定化。在光照下类囊体膜发生光合作用的过程中，水被氧化分离。当阳极侧释放氧气时，阴极侧通过生物电催化方法将氧气还原为水。细胞色素C用于类囊体膜与电极表面的结合，在光合作用的释放电子的转移中起着重要作用。