The postmortem calpain-11 role in ostrich muscle was investigated. Pairs of ostrich muscle (Iliotibialis cranialis) were excised from 32 ostrich carcasses in 3-h postmortem and randomly assigned into four treatments. The muscle was cut into 2.5-cm thick meat cores. The cores were incubated in 30 mM CaCl₂, 30 mM EDTA, 90 mM NaCl, or control. The cores from the left-side carcasses were sampled after 0, 1, 2, and 3 days of incubation at 5 °C, while the right-side meat cores were taken at 1-day and 3-day incubation for shear force measurements. The results showed that the decrease in unautolyzed and total activities of calpain-11, desmin content and shear force was more rapid in CaCl₂-incubated samples than in control, NaCl- and EDTA-incubated samples. Thus, present results suggest that in the absence of calpain-1, calpain-11 with an extensive activation by adding exogenous Ca²⁺ could enhance the postmortem proteolysis and tenderization of ostrich muscle.

研究了死后钙蛋白酶11在鸵鸟肌肉中的作用。在死后3小时内从32个鸵鸟尸体中切除成对的鸵鸟肌肉（Iliotibialis cranialis），并随机分配给4种处理方法。将肌肉切成2.5厘米厚的肉芯。芯在30毫米氯化钙孵育₂，30mM的EDTA，90mM的氯化钠，或控制。在5°C下孵育0、1、2和3天后，对左侧屠体的核心进行采样，而在1天和3天的孵育中采集右侧肉核心以测量剪切力。结果表明，CaCl ₂中钙蛋白酶11的自溶和总活性，结蛋白含量和剪切力的下降较快。-温育的样品比对照，NaCl和EDTA温育的样品高。因此，目前的结果表明，在没有钙蛋白酶1的情况下，通过添加外源Ca ²⁺而具有广泛活化的钙蛋白酶11可以增强鸵鸟肌肉的死后蛋白水解和嫩化。