We present an open-source, extensible search engine for shotgun proteomics. Implemented in Python programming language, IdentiPy shows competitive processing speed and sensitivity compared with the state-of-the-art search engines. It is equipped with a user-friendly web interface, IdentiPy Server, enabling the use of a single server installation accessed from multiple workstations. Using a simplified version of X!Tandem scoring algorithm and its novel “autotune” feature, IdentiPy outperforms the popular alternatives on high-resolution data sets. Autotune adjusts the search parameters for the particular data set, resulting in improved search efficiency and simplifying the user experience. IdentiPy with the autotune feature shows higher sensitivity compared with the evaluated search engines. IdentiPy Server has built-in postprocessing and protein inference procedures and provides graphic visualization of the statistical properties of the data set and the search results. It is open-source and can be freely extended to use third-party scoring functions or processing algorithms and allows customization of the search workflow for specialized applications.

中文翻译：

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IdentiPy：用于Shot弹枪蛋白质组学中蛋白质鉴定的可扩展搜索引擎

我们提供了一种用于散弹枪蛋白质组学的开源可扩展搜索引擎。与最先进的搜索引擎相比，IdentiPy以Python编程语言实现，显示出具有竞争力的处理速度和敏感性。它配备了用户友好的Web界面IdentiPy Server，可使用从多个工作站访问的单个服务器安装。使用X！Tandem评分算法的简化版本及其新颖的“自动调谐”功能，IdentiPy优于高分辨率数据集上流行的替代方法。自动调谐会针对特定数据集调整搜索参数，从而提高搜索效率并简化用户体验。与经过评估的搜索引擎相比，具有自动调谐功能的IdentiPy显示出更高的灵敏度。IdentiPy Server具有内置的后处理和蛋白质推论程序，并提供图形化的可视化数据集和搜索结果的统计属性。它是开源的，可以自由扩展以使用第三方评分功能或处理算法，并允许针对特定应用程序定制搜索工作流程。