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Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating.
Langmuir ( IF 3.9 ) Pub Date : 2018-04-23 , DOI: 10.1021/acs.langmuir.8b00181
Christopher Ziemba 1 , Maria Khavkin 1 , Dimitris Priftis 2 , Handan Acar 2 , Jun Mao 2 , Maya Benami 1 , Moshe Gottlieb 3 , Matthew Tirrell 2, 4 , Yair Kaufman 1 , Moshe Herzberg 1
Affiliation  

There is a need for the development of antifouling materials to resist adsorption of biomacromolecules. Here we describe the preparation of a novel zwitterionic block copolymer with the potential to prevent or delay the formation of microbial biofilms. The block copolymer comprised a zwitterionic (hydrophilic) section of alternating glutamic acid (negatively charged) and lysine (positively charged) units and a hydrophobic polystyrene section. Cryo-TEM and dynamic-light-scattering (DLS) results showed that, on average, the block copolymer self-assembled into 7-nm-diameter micelles in aqueous solutions (0 to 100 mM NaCl, pH 6). Quartz crystal microbalance with dissipation monitoring (QCM-D), atomic force microscopy (AFM), and contact angle measurements demonstrated that the block copolymer self-assembled into a brush-like monolayer on polystyrene surfaces. The brush-like monolayer produced from a 100 mg/L block copolymer solution exhibited an average distance, d, of approximately 4-8 nm between each block copolymer molecule (center to center). Once the brush-like monolayer self-assembled, it reduced EPS adsorption onto the polystyrene surface by ∼70% (mass), reduced the rate of bacterial attachment by >80%, and inhibited the development of thick biofilms. QCM-D results revealed that the EPS molecules penetrate between the chains of the brush and adsorb onto the polystyrene surface. Additionally, AFM analyses showed that the brush-like monolayer prevents the adhesion of large (> d) hydrophilic colloids onto the surface via hydration repulsion; however, molecules or colloids small enough to fit between the brush polymers (< d) were able to be adsorbed onto the surface via van der Waals interactions. Overall, we found that the penetration of extracellular organelles, as well as biopolymers through the brush, is critical for the failure of the antifouling coating, and likely could be prevented through tuning of the brush density. Stability and biofilm development testing on multiple surfaces (polypropylene, glass, and stainless steel) support practical applications of this novel block copolymer.

中文翻译:

自组装的谷氨酸-赖氨酸两性离子聚合物表面涂层的防污性能。

需要开发防污材料以抵抗生物大分子的吸附。在这里,我们描述了一种新型的两性离子嵌段共聚物的制备,该共聚物具有防止或延迟微生物生物膜形成的潜力。该嵌段共聚物包括交替的谷氨酸(带负电荷)和赖氨酸(带正电荷)单元的两性离子(亲水)部分和疏水性聚苯乙烯部分。低温TEM和动态光散射(DLS)结果表明,嵌段共聚物平均在水溶液(0至100 mM NaCl,pH 6)中自组装成直径为7 nm的胶束。带有耗散监控(QCM-D),原子力显微镜(AFM)的石英晶体微量天平,接触角的测量和结果表明,嵌段共聚物在聚苯乙烯表面上自组装成刷状单层。由100 mg / L嵌段共聚物溶液制得的刷状单层在每个嵌段共聚物分子之间(中心到中心)的平均距离d约为4-8 nm。一旦刷状单层自组装,它就将EPS吸附到聚苯乙烯表面的质量降低了约70%(质量),细菌附着率降低了80%以上,并抑制了厚厚的生物膜的形成。QCM-D结果表明,EPS分子渗透到刷子的链之间,并吸附到聚苯乙烯表面上。此外,原子力显微镜分析表明,刷状单分子层可防止大量(> d)亲水胶体通过水合作用排斥在表面上。然而,足够小以适合刷状聚合物(<d)之间的分子或胶体能够通过范德华相互作用被吸附到表面上。总的来说,我们发现细胞外细胞器以及生物聚合物通过刷子的渗透对于防污涂层的失效至关重要,并且很可能可以通过调节刷子的密度来防止。在多个表面(聚丙烯,玻璃和不锈钢)上的稳定性和生物膜显影测试支持了这种新型嵌段共聚物的实际应用。对于防污涂层的失效至关重要,并且很可能可以通过调整刷子的密度来防止。在多个表面(聚丙烯,玻璃和不锈钢)上的稳定性和生物膜显影测试支持了这种新型嵌段共聚物的实际应用。对于防污涂层的失效至关重要,并且很可能可以通过调整刷子的密度来防止。在多个表面(聚丙烯,玻璃和不锈钢)上的稳定性和生物膜显影测试支持了这种新型嵌段共聚物的实际应用。
更新日期:2018-04-11
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