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Exploring drying pattern of a sessile droplet of genomic DNA in the presence of hematite nanoparticles.
Scientific Reports ( IF 4.6 ) Pub Date : 2018-Apr-20 , DOI: 10.1038/s41598-018-24821-1
Rekha Bhar , Gurpreet Kaur , S. K. Mehta

For the first time, drying pattern of a sessile droplet of genomic DNA, in the presence of hematite nanoparticles was sighted by polarizing optical microscopy (POM) in this research article. POM results indicated that only at an appreciably high concentration of hematite nanoparticles dried pattern of deoxyribonucleic acid from calf thymus (CT-DNA) was altered. Iron hybridized cetylpyridinium chloride was utilized for the preparation of iron oxide nanoparticles through hydrothermal method. Fourier transforms infrared spectroscopy (FTIR) and powder x-ray diffraction (PXRD) studies confirmed the formation of highly crystalline hematite i.e. α-Fe2O3 nanoparticles. Morphology of the synthesized nanoparticle was visualized by transmission electron microscope (TEM) and field emission scanning electron microscope (FESEM), which revealed that nanoparticles were rhombohedral in shape with a size of 45 ± 10 nm. Based upon all the findings, hydrothermal growth mechanism was also proposed having bilayer protection of surfactant around the nanoparticles. UV-Vis spectroscopy and fluorescence spectroscopy were explored to study the affinity of thus prepared nanoparticles towards calf thymus deoxyribonucleic acid (CT-DNA). The low value of binding constant calculated from the spectroscopy data confirmed the weak interaction between nanoparticles and the CT-DNA.

中文翻译:

探索在赤铁矿纳米粒子存在下基因组DNA固着小滴的干燥模式。

本文首次通过偏光光学显微镜(POM)观察到在赤铁矿纳米粒子存在下,基因组DNA固着小滴的干燥模式。POM结果表明,仅在相当高浓度的赤铁矿纳米颗粒中,来自小牛胸腺的脱氧核糖核酸(CT-DNA)的干燥模式就会改变。利用铁杂化十六烷基吡啶氯化钠通过水热法制备了氧化铁纳米颗粒。傅立叶变换红外光谱(FTIR)和粉末X射线衍射(PXRD)研究证实的高度结晶的赤铁矿即的α-Fe的形成2 ö 3纳米粒子。合成的纳米颗粒的形态通过透射电子显微镜(TEM)和场发射扫描电子显微镜(FESEM)观察,显示纳米颗粒呈菱形,尺寸为45±10nm。基于所有发现,还提出了水热生长机理,其在纳米颗粒周围具有表面活性剂的双层保护。探索紫外-可见光谱和荧光光谱以研究如此制备的纳米颗粒对小牛胸腺脱氧核糖核酸(CT-DNA)的亲和力。由光谱数据计算的低的结合常数值证实了纳米颗粒与CT-DNA之间的弱相互作用。
更新日期:2018-04-20
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