Ion mobility mass spectrometry (IM-MS) holds great potential for structural glycobiology, in particular in its ability to resolve glycan isomers. Generally, IM-MS has largely been applied to intact glycoconjugate ions with reports focusing on the separation of different adduct types. Here, we explore IM separation and report the collision cross section (CCS) of complex type N-glycans and their fragments in negative ion mode following collision-induced dissociation (CID). CCSs of isomeric fragment ions were found, in some cases, to reveal structural details that were not present in CID spectra themselves. Many fragment ions were confirmed as possessing multiple structure, details of which could be obtained by comparing their drift time profiles to different glycans. By using fragmentation both before and after mobility separation, information was gathered on the fragmentation pathways producing some of the ions. These results help demonstrate the utility of IM and will contribute to the growing use of IM-MS for glycomics.

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离子淌度质谱（IM-MS）在结构糖生物学方面具有巨大潜力，尤其是在分辨聚糖异构体方面。通常，IM-MS已广泛应用于完整的糖缀合物离子，其报道集中在不同加合物类型的分离上。在这里，我们探索IM分离，并在碰撞诱导解离（CID）后以负离子模式报告复杂类型N-聚糖及其片段的碰撞截面（CCS）。在某些情况下，发现同分异构碎片离子的CCS揭示了CID光谱本身不存在的结构细节。已确认许多碎片离子具有多重结构，可通过将其漂移时间曲线与不同聚糖进行比较来获得其详细信息。通过在迁移率分离之前和之后使用碎片化，收集了有关产生某些离子的裂解途径的信息。这些结果有助于证明IM的实用性，并将有助于IM-MS越来越多地用于糖组学。

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