Moderate leaf rolling is important in ideotype breeding, as it improves photosynthetic efficiency and therefore increases crop yields. To understand the regulatory network of leaf rolling in Brassica napus, a down-curved leaf mutant (Bndcl1) has been investigated. Physiological analyses indicated that the chlorophyll contents and antioxidant enzyme activities were remarkably increased and the photosynthetic performance was significantly improved in Bndcl1. Consistent with these findings, 943 differentially accumulated proteins (DAPs) were identified in the Bndcl1 mutant and its wild-type plants using iTRAQ-based comparative proteomic analyses. Enrichment analysis of proteins with higher abundance in Bndcl1 revealed that the functional category “photosynthesis” was significantly overrepresented. Moreover, proteins associated with oxidative stress response and photosystem II repairing were also up-accumulated in Bndcl1, which might help the mutant to sustain the photosynthetic efficiency under unfavorable conditions. Histological observation showed that the mutant displayed defects in adaxial-abaxial patterning. Important DAPs associated with leaf polarity establishment were detected in Bndcl1, including ribosomal proteins, proteins involved in post-transcriptional gene silencing, and proteins related to brassinosteroid. Together, our findings may help clarify the mechanisms underlying leaf rolling and its physiological effects on plants and may facilitate ideotype breeding in Brassica napus.

中文翻译：

---

组织学，生理学和比较蛋白质组学分析为甘蓝型油菜的叶片滚动提供了见识

适度的卷叶在同型育种中很重要，因为它提高了光合效率，因此增加了农作物的产量。为了了解甘蓝型油菜叶片滚动的调控网络，已经研究了向下弯曲的叶片突变体（Bndcl1）。生理分析表明，Bndcl1的叶绿素含量和抗氧化酶活性显着提高，光合性能显着提高。与这些发现一致，使用基于iTRAQ的比较蛋白质组学分析，在Bndcl1突变体及其野生型植物中鉴定出943个差异累积蛋白（DAP）。Bndcl1中高丰度蛋白质的富集分析揭示了功能类别“光合作用”的代表过多。此外，与氧化应激反应和光系统II修复相关的蛋白质也在Bndcl1中积累，这可能有助于突变体在不利条件下维持光合作用效率。组织学观察表明该突变体在前后轴构图中显示出缺陷。在Bndcl1中检测到了与叶片极性建立相关的重要DAP ，包括核糖体蛋白，转录后基因沉默中涉及的蛋白以及与油菜素类固醇有关的蛋白。总之，我们的发现可能有助于弄清叶卷起的机理及其对植物的生理影响，并可能有助于在水稻中进行表型育种。甘蓝型油菜。