Herein, we report a carbazole (Cz) ligand that displays distinct turn-on fluorescence signals upon interaction with human telomeric G-quadruplex (h-TELO) and nuclease enzymes. Interestingly, Cz selectively binds and stabilizes the mixed hybrid topology of h-TELO G-quadruplex that withstands digestion by exonucleases and nuclease S1. The distinct fluorescence signatures of Cz-stabilized h-TELO with nucleases are used to design conceptually novel DNA devices for selectively detecting the enzymatic activity of DNase I as well as performing logic operations. An INHIBIT logic gate is constructed using h-TELO and DNase I as the inputs while the inputs of h-TELO and nuclease S1 form a YES logic gate. Furthermore, a two-input two-output reusable logic device with “multireset” function is developed by using h-TELO and DNase I as inputs. On the basis of this platform, combinatorial logic systems (INHIBIT–INHIBIT and NOR–OR) have been successfully installed using different combinations of nucleases as inputs. Moreover, this new strategy of using a synthetic dual emissive probe and enzyme/DNA inputs for constructing reusable logic device may find important applications in biological computing and information processing.

中文翻译：

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酶调节的基于DNA的逻辑设备

在本文中，我们报告了咔唑（Cz）配体，该配体在与人端粒G-四链体（h-TELO）和核酸酶相互作用后显示出明显的开启荧光信号。有趣的是，Cz选择性地结合并稳定了h-TELO G-四链体的混合杂交拓扑结构，该拓扑结构可承受核酸外切酶和核酸酶S1的消化作用。Cz稳定的h-TELO与核酸酶的独特荧光特征被用于设计概念上新颖的DNA装置，用于选择性检测DNase I的酶活性并执行逻辑运算。使用h-TELO和DNase I作为输入构建一个INHIBIT逻辑门，而将H-TELO和DNase I作为输入构建h-TELO和核酸酶S1形成“是”逻辑门。此外，通过使用h-TELO和DNase I作为输入，开发了具有“多重复位”功能的两输入两输出可重用逻辑器件。在此平台的基础上，已经成功使用不同的核酸酶组合作为输入成功安装了组合逻辑系统（INHIBIT–INHIBIT和NOR–OR）。此外，这种使用合成的双发射探针和酶/ DNA输入来构建可重复使用的逻辑设备的新策略可能会在生物计算和信息处理中找到重要的应用。