当前位置: X-MOL 学术Sci. Adv. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Noninvasive ovarian cancer biomarker detection via an optical nanosensor implant.
Science Advances ( IF 13.6 ) Pub Date : 2018-Apr-01 , DOI: 10.1126/sciadv.aaq1090
Ryan M. Williams 1 , Christopher Lee 1 , Thomas V. Galassi 1, 2 , Jackson D. Harvey 1, 2 , Rachel Leicher 3, 4 , Maria Sirenko 1 , Madeline A. Dorso 1, 2 , Janki Shah 1 , Narciso Olvera 5 , Fanny Dao 5 , Douglas A. Levine 5 , Daniel A. Heller 1, 2
Affiliation  

Patients with high-grade serous ovarian carcinoma (HGSC) exhibit poor 5-year survival rates, which may be significantly improved by early-stage detection. The U.S. Food and Drug Administration-approved biomarkers for HGSC-CA-125 (cancer antigen 125) and HE4 (human epididymis protein 4)-do not generally appear at detectable levels in the serum until advanced stages of the disease. An implantable device placed proximal to disease sites, such as in or near the fallopian tube, ovary, uterine cavity, or peritoneal cavity, may constitute a feasible strategy to improve detection of HGSC. We engineered a prototype optical sensor composed of an antibody-functionalized carbon nanotube complex, which responds quantitatively to HE4 via modulation of the nanotube optical bandgap. The complexes measured HE4 with nanomolar sensitivity to differentiate disease from benign patient biofluids. The sensors were implanted into four models of ovarian cancer, within a semipermeable membrane, enabling the optical detection of HE4 within the live animals. We present the first in vivo optical nanosensor capable of noninvasive cancer biomarker detection in orthotopic models of disease.

中文翻译:

通过光学纳米传感器植入物进行非侵入性卵巢癌生物标志物检测。

高度浆液性卵巢癌(HGSC)患者的5年生存率较差,早期检测可显着改善。HGSC-CA-125(癌症抗原125)和HE4(人附睾蛋白4)的美国食品药品监督管理局批准的生物标记通常在疾病晚期才以可检测的水平出现在血清中。放置在疾病部位附近的可植入设备,例如输卵管,卵巢,子宫腔或腹膜腔内或附近,可能构成改善HGSC检测的可行策略。我们设计了一种由抗体功能化的碳纳米管复合物组成的原型光学传感器,该传感器通过调节纳米管光学带隙对HE4进行定量反应。该复合物以纳摩尔浓度检测HE4,以区分疾病与良性患者生物流体。传感器被植入半透膜内的四种卵巢癌模型中,从而可以对活体动物中的HE4进行光学检测。我们提出了第一个能够在疾病原位模型中进行非侵入性癌症生物标志物检测的体内光学纳米传感器。
更新日期:2018-04-19
down
wechat
bug