Metabolomics, as a promising and powerful approach, refers to comprehensive assessment and identification of small molecule endogenous metabolites in a biological system which is capable of further understanding the mechanisms of diseases for early diagnosis, effective treatment and prognosis. Acute kidney injury (AKI) induced by contrast is a serious complication in patients undergoing administration of iodinated contrast media. It is becoming a major health concern in clinic, however, the molecular mechanisms of contrast-induced acute kidney injury (CI-AKI) have not been well characterized. In this study, we used serum metabolomics based on liquid chromatography-mass spectrometry (LC-MS) combined with pattern recognition to explore and characterize potential metabolites and metabolic pathway in an experimental model for CI-AKI. Seventeen differentiating metabolites in the serum were identified involving the pivotal metabolic pathways related to tryptophan metabolism, glycerophospholipid metabolism, steroid hormone biosynthesis, pyrimidine metabolism, sphingolipid metabolism, aminoacyl-tRNA biosynthesis. Our study provides novel insight into pathophysiologic mechanisms of AKI by changing biomarkers and pathways.

中文翻译：

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高通量代谢组学利用 LC-MS 结合模式识别方法识别急性肾损伤的血清代谢特征

代谢组学是一种有前途且强大的方法，是指对生物系统中小分子内源代谢物进行全面评估和鉴定，能够进一步了解疾病的机制，从而实现早期诊断、有效治疗和预后。造影剂引起的急性肾损伤（AKI）是接受碘造影剂治疗的患者的严重并发症。它正在成为临床上的一个主要健康问题，然而，造影剂诱发的急性肾损伤（CI-AKI）的分子机制尚未得到很好的表征。在本研究中，我们利用基于液相色谱-质谱联用（LC-MS）结合模式识别的血清代谢组学来探索和表征 CI-AKI 实验模型中潜在的代谢物和代谢途径。血清中鉴定出 17 种差异代谢物，涉及与色氨酸代谢、甘油磷脂代谢、类固醇激素生物合成、嘧啶代谢、鞘脂代谢、氨酰基-tRNA 生物合成相关的关键代谢途径。我们的研究通过改变生物标志物和通路，为 AKI 的病理生理机制提供了新的见解。