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A methylene blue-based near-infrared fluorescent probe for rapid detection of hypochlorite in tap water and living cells†
RSC Advances ( IF 3.9 ) Pub Date : 2018-04-18 00:00:00 , DOI: 10.1039/c8ra01037d
Xin Huang 1 , Zhipeng Li 1 , Tingting Cao 1 , Qian Cai 1 , Chengchu Zeng 1 , Hua Fu 2 , Liming Hu 1, 2
Affiliation  

A methylene blue-based near-infrared fluorescent probe was designed for the selective determination of hypochlorite (ClO), over other reactive oxygen species or interfering agents. Acetylated methylene blue was synthesized by introducing the acetyl group into the methylene blue framework, which can specifically recognize exogenous and endogenous ClO. The acetylated methylene blue fluorescent probe was characterized by 1H NMR, 13C NMR and HRMS. The response process and possible mechanism were studied using products of the probe. The emission response of the probe to ClO presented good linear relationship in the 0–60 μM concentration range, with the detection limit of 0.1 μM (measured at 660 nm and 690 nm). The absorption and emission wavelengths of acetylated methylene blue are both in the near-infrared region; in addition, the probe itself and the degradation products were well-dissolved in water and have almost no toxicity. The probe was used for intracellular ClO imaging and showed a large fluorescence enhancement (about 200-fold increase).

中文翻译:

一种基于亚甲基蓝的近红外荧光探针,用于快速检测自来水和活细胞中的次氯酸盐†

基于亚甲蓝的近红外荧光探针设计用于选择性测定次氯酸盐 (ClO - ),而不是其他活性氧或干扰剂。通过将乙酰基引入亚甲蓝骨架中合成乙酰化亚甲蓝,可特异性识别外源性和内源性ClO -。乙酰化亚甲基蓝荧光探针通过1 H NMR、13 C NMR和HRMS表征。使用探针产品研究了响应过程和可能的机制。探针对 ClO 的发射响应-在 0-60 μM 浓度范围内呈现良好的线性关系,检测限为 0.1 μM(在 660 nm 和 690 nm 处测量)。乙酰化亚甲蓝的吸收和发射波长均在近红外区;此外,探针本身和降解产物都很好地溶解在水中,几乎没有毒性。该探针用于细胞内ClO -成像并显示出较大的荧光增强(增加约200倍)。
更新日期:2018-04-18
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