In this paper, we present a new strategy for microprinting dense bacterial communities with a prescribed organization on a substrate. Unlike conventional bioprinting techniques that require bioinks, through optogenetic manipulation, we directly manipulated the behaviors of Pseudomonas aeruginosa to allow these living bacteria to autonomically form patterned biofilms following prescribed illumination. The results showed that through optogenetic manipulation, patterned bacterial communities with high spatial resolution (approximately 10 μm) could be constructed in 6 h. Thus, optogenetic manipulation greatly increases the range of available bioprinting techniques.

中文翻译：

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通过光遗传学操作对活生物膜进行生物印刷

在本文中，我们提出了一种新的策略，可以在基底上使用指定的组织对密集的细菌群落进行微打印。与需要生物墨水的常规生物打印技术不同，通过光遗传学操作，我们直接控制了铜绿假单胞菌的行为，以使这些活细菌在规定的照明条件下自主形成带图案的生物膜。结果表明，通过光遗传学操作，可以在6小时内构建具有高空间分辨率（约10μm）的模式化细菌群落。因此，光遗传学操作大大增加了可用的生物打印技术的范围。