Proper sample preparation in proteomic workflows is essential to the success of modern mass spectrometry experiments. Complex workflows often require reagents which are incompatible with MS analysis (e.g., detergents) necessitating a variety of sample cleanup procedures. Efforts to understand and mitigate sample contamination are a continual source of disruption with respect to both time and resources. To improve the ability to rapidly assess sample contamination from a diverse array of sources, I developed a molecular library in Skyline for rapid extraction of contaminant precursor signals using MS1 filtering. This contaminant template library is easily managed and can be modified for a diverse array of mass spectrometry sample preparation workflows. Utilization of this template allows rapid assessment of sample integrity and indicates potential sources of contamination.

中文翻译：

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使用Skyline快速评估质谱数据中的污染物和干扰

蛋白质组学工作流程中正确的样品制备对于现代质谱实验的成功至关重要。复杂的工作流程通常需要与MS分析不兼容的试剂（例如去污剂），从而需要各种样品净化程序。理解和减轻样品污染的努力是持续的时间和资源中断源。为了提高快速评估来自各种来源的样品污染的能力，我在Skyline中开发了分子库，可使用MS1过滤快速提取污染物前体信号。该污染物模板库易于管理，可以针对各种质谱样品制备工作流程进行修改。