Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures†,Chemical Communications

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Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures†
Chemical Communications ( IF 4.9 ) Pub Date : 2018-04-17 00:00:00 , DOI: 10.1039/c8cc00738a
Ilenia Manuguerra _{1,

2,

3,

4,

5} , Stefano Croce _{6,

7,

8,

9,

10} , Afaf H. El-Sagheer _{7,

11,

12,

13,

14} , Abhichart Krissanaprasit _{1,

2,

3,

4,

5} , Tom Brown _{7,

11,

12,

13,

14} , Kurt V. Gothelf _{1,

2,

3,

4,

5} , Antonio Manetto _{10,

15,

16}

Affiliation

Interdisciplinary Nanoscience Center (iNANO)
Gustav Wieds Vej 14 and Department of Chemistry
Aarhus University
8000 Aarhus
Denmark
Center for Integrated Protein Science
Department of Chemistry
Ludwig-Maximilians-Universität (LMU)
81377 Munich
Germany
University of Oxford
Chemistry Research Laboratory
Oxford
UK
Metabion GmbH
82152 Planegg Munich

Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.

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