Bioorthogonal chemistry has found increased application in living systems over the past decade. In particular, tetrazine bioorthogonal chemistry has become a powerful tool for imaging, detection, and diagnostic purposes, as reflected in the increased number of examples reported in the literature. The popularity of tetrazine ligations are likely due to rapid and tunable kinetics, the existence of high quality fluorogenic probes, and the selectivity of reaction. In this Account, we summarize our recent efforts to advance tetrazine bioorthogonal chemistry through improvements in synthetic methodology, with an emphasis on developing new routes to tetrazines and expanding the range of useful dienophiles. These efforts have removed specific barriers that previously limited tetrazine ligations and have broadened their potential applications.

中文翻译：

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新型Tetrazines和Dienophiles的合成驱动Tetrazine生物正交化学的研究进展

在过去的十年中，生物正交化学已在生物系统中得到越来越多的应用。特别是，四嗪生物正交化学已成为用于成像，检测和诊断目的的强大工具，正如文献中报道的越来越多的实例所反映的那样。四嗪连接的流行可能是由于快速且可调的动力学，高质量荧光探针的存在以及反应的选择性。在此报告中，我们总结了我们最近通过改进合成方法来推进四嗪生物正交化学的工作，重点是开发四嗪的新途径并扩大了有用的亲二烯体的范围。这些努力消除了以前限制四嗪连接的特定障碍，并拓宽了其潜在的应用范围。