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Lactoferrin preserves bone homeostasis by regulating the RANKL/RANK/OPG pathway of osteoimmunology
Food & Function ( IF 6.1 ) Pub Date : 2018-04-11 00:00:00 , DOI: 10.1039/c8fo00303c
Fengjiao Fan 1, 2, 3, 4, 5 , Pujie Shi 1, 2, 3, 4 , Meng Liu 1, 2, 3, 4 , Hui Chen 4, 5, 6, 7, 8 , Maolin Tu 1, 2, 3, 4 , Weihong Lu 1, 2, 3, 4 , Ming Du 1, 2, 3, 4, 5
Affiliation  

Osteoporosis can be classified as an inflammatory disease and the crosstalk between the immune system and bone growth should be considered. The effects of bovine lactoferrin on bone by osteoimmunology were investigated in the present study. Ten week old female BALB/c mice were ovariectomized (OVX) and fed for 12 weeks with a control diet or lactoferrin (2, 20 and 100 mg kg−1 d−1). The results showed that following 12 weeks of treatment after surgery, OVX resulted in bone loss, but treatment with lactoferrin preserved bone homeostasis. Lactoferrin significantly improved bone mineral density, and increased the serum levels of alkaline phosphatase, while it decreased the serum levels of tartrate-resistant acid phosphatase. Furthermore, according to micro-computed tomography (micro-CT), the bone volume per tissue volume (BV/TV), trabecular thickness (Tb.Th) and trabecular number (Tb.N) were elevated. The results indicated that the structure model index (SMI) was reduced in the OVX + LF groups. Additionally, lactoferrin enhanced the Max-Load and Max-Stress values of the femur, and increased the content of Ca and P. However, lactoferrin suppressed the RANKL/OPG ratio in OVX mice. Moreover, interferon-γ, interleukin-5 and interleukin-10 were elevated significantly in the OVX + LF groups. Lactoferrin had a positive effect on the bone micro-environment and might be a pleiotropic protein for the prevention and treatment of estrogen-dependent bone loss via the osteoimmunology pathway.

中文翻译:

乳铁蛋白通过调节骨免疫学的RANKL / RANK / OPG途径来保持骨稳态

骨质疏松症可归为炎性疾病,应考虑免疫系统与骨骼生长之间的串扰。在本研究中,研究了牛乳铁蛋白对骨的影响。将十周大的雌性BALB / c小鼠切除卵巢(OVX),并用对照饮食或乳铁蛋白(2、20和100 mg kg -1 d -1喂养12周))。结果表明,在术后12周的治疗中,OVX导致骨质流失,但乳铁蛋白治疗可保持骨稳态。乳铁蛋白可显着改善骨矿物质密度,并增加血清碱性磷酸酶水平,同时降低血清抗酒石酸酸性磷酸酶水平。此外,根据显微计算机断层摄影术(micro-CT),提高了每组织体积的骨量(BV / TV),小梁厚度(Tb.Th)和小梁数目(Tb.N)。结果表明,在OVX + LF组中,结构模型索引(SMI)降低了。此外,乳铁蛋白提高了股骨的最大负荷和最大应力值,并增加了钙和磷的含量。但是,乳铁蛋白抑制了OVX小鼠的RANKL / OPG比。而且,γ-干扰素 OVX + LF组白细胞介素5和白细胞介素10显着升高。乳铁蛋白对骨骼微环境有积极作用,可能是预防和治疗雌激素依赖性骨质流失的多效蛋白通过骨免疫学途径。
更新日期:2018-04-11
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