Background

Non-alcoholic fatty liver disease (NAFLD) is associated with hyperlipidemia, obesity and type II diabetes. Due to increasing prevalence of these diseases globally, NAFLD is considered as a common form of chronic liver diseases. Vitamin D is a fat soluble vitamin with reported anti-inflammatory, anti-oxidant and immune modulating activity. Hypovitaminosis D often coexists with NAFLD and various studies reported beneficial role of vitamin D in modulating NAFLD. However, variable oral bioavailability, poor water solubility, and chemical degradation hinder the clinical application of vitamin D.

Purpose

We evaluated the potential protective effect of Vitamin D nanoemulsion (developed by sonication and pH-Shifting of pea protein isolate and canola oil) compared to conventional vitamin D against liver injury in rats fed with high fat diet (HFD).

Methods

We analyzed liver function enzymes, lipid profile, lipid metabolism, levels and histopathology of inflammation and fibrosis in rat liver tissues.

Results

HFD fed rats exhibited deterioration of liver function, poor lipid profile, decreased fatty acid oxidation and up-regulation of inflammatory cytokines and extracellular matrix deposition. Vitamin D administration reduced elevated liver enzymes, improved lipid profile, enhanced fatty acid oxidation and attenuated liver inflammation and fibrosis. Interestingly, vitamin D nanoemulsion was superior to conventional vitamin D with remarkable hepatoprotective effect against HFD-induced liver injury.

Conclusion

This study demonstrated vitamin D nanoemulsion as a more efficient formulation with more prominent hepatoprotective effect against HFD-induced liver injury compared to conventional oral vitamin D.

中文翻译：

---

维生素D纳米乳剂可增强高脂饮食喂养的大鼠体内常规维生素D的防菌功效

背景

非酒精性脂肪肝疾病（NAFLD）与高脂血症，肥胖症和II型糖尿病有关。由于全球范围内这些疾病的患病率上升，NAFLD被认为是慢性肝病的一种常见形式。维生素D是一种脂溶性维生素，具有抗炎，抗氧化和免疫调节活性。低维生素D经常与NAFLD共存，各种研究报道了维生素D在调节NAFLD中的有益作用。然而，可变的口服生物利用度，差的水溶性和化学降解阻碍了维生素D的临床应用。

目的

我们评估了维生素D纳米乳剂（通过对豌豆蛋白分离物和低芥酸菜子油进行超声处理和pH改变而开发的）与常规维生素D相比对高脂饮食（HFD）喂养的大鼠肝损伤的潜在保护作用。

方法

我们分析了大鼠肝组织中炎症功能和纤维化的肝功能酶，脂质分布，脂质代谢，水平和组织病理学。

结果

用HFD喂养的大鼠表现出肝功能恶化，脂质状况不佳，脂肪酸氧化降低以及炎性细胞因子和细胞外基质沉积的上调。维生素D的施用减少了肝酶的升高，改善了脂质分布，增强了脂肪酸的氧化，并减轻了肝脏的炎症和纤维化。有趣的是，维生素D纳米乳剂优于常规维生素D，对HFD引起的肝损伤具有显着的保肝作用。

结论

这项研究表明，与常规口服维生素D相比，维生素D纳米乳剂是一种更有效的配方，对HFD引起的肝损伤具有更显着的保肝作用。