In order to explore whether the acidogenic phase and methanogenic phase could be separated vertically into a single-stage anaerobic reactor, a controlled double circulation (CDC) anaerobic reactor was proposed for treating traditional Chinese medicine (TCM) wastewater in this study. The results showed that most of the organic pollutants and refractory were removed in the first reaction area where most of the amount of sludge existed. The organic acids were accumulated in the first reaction area, and larger specific methanogenic activity (SMA) and coenzyme F₄₂₀ values were found in the second reaction area. Bacterial and archaeal community structures in the two reaction areas of the CDC reactor were analysised by Illumina MiSeq Sequencing, which revealed that the archaeal community showed larger difference compared with the bacterial community. Differences in the performance and microbial composition of the two reaction areas confirmed that phase separation was implemented in the CDC reactor.

为了探讨产酸相和产甲烷相是否可以垂直分离成单级厌氧反应器，本研究提出了一种可控双循环厌氧反应器来处理中药废水。结果表明，在存在大量污泥的第一反应区，大部分有机污染物和耐火材料被去除。有机酸积累在第一反应区，并具有较大的产甲烷比活性（SMA）和辅酶F ₄₂₀在第二反应区发现了值。通过Illumina MiSeq Sequencing对CDC反应器两个反应区的细菌和古细菌群落结构进行了分析，结果表明，与细菌群落相比，古细菌群落的差异更大。两个反应区域的性能和微生物组成的差异证实了在CDC反应器中进行了相分离。