Controlled conjugation of fluorescent carbon dots (CDs) with DNA and subsequent fabrication of the CDs into an array through hybridization mediated self-assembly in the solution phase is reported. Covalent conjugation of CD with DNA and the subsequent array formation change the mobility of the CD-DNA array in gel electrophoresis and HPLC significantly. Interspatial distance in the CD-DNA array is tuned by the DNA sequence length and maintained at ∼8 ± 0.3 nm as revealed by electron microscopy studies. An increase in fluorescence lifetime by ∼2 ns was observed for the CD-DNA array compared to a solitary CD, vis-á-vis better imaging prospects of HEK293 cells by the former. Thus, the array displays improved fluorescence and unhindered cell penetration.

中文翻译：

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通过与DNA的共价共轭缀合，创建具有改善的光学性质的线性碳点阵列。

报告了荧光碳点（CD）与DNA的控制共轭，以及随后在溶液相中通过杂交介导的自组装将CD制成阵列的过程。CD与DNA的共价缀合以及随后的阵列形成极大地改变了CD-DNA阵列在凝胶电泳和HPLC中的迁移率。CD-DNA阵列中的空间间距可通过DNA序列长度进行调整，并保持在〜8±0.3 nm，如电子显微镜研究所揭示。与单独的CD相比，CD-DNA阵列的荧光寿命增加了约2 ns，而前者对HEK293细胞的成像前景更好。因此，该阵列显示出改善的荧光和不受阻碍的细胞渗透。