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Probing Ion Channel Structure and Function Using Light-Sensitive Amino Acids
Trends in Biochemical Sciences ( IF 13.8 ) Pub Date : 2018-04-10
Viktoria Klippenstein, Laetitia Mony, Pierre Paoletti

Approaches to remotely control and monitor ion channel operation with light are expanding rapidly in the biophysics and neuroscience fields. A recent development directly introduces light sensitivity into proteins by utilizing photosensitive unnatural amino acids (UAAs) incorporated using the genetic code expansion technique. The introduction of UAAs results in unique molecular level control and, when combined with the maximal spatiotemporal resolution and poor invasiveness of light, enables direct manipulation and interrogation of ion channel functionality. Here, we review the diverse applications of light-sensitive UAAs in two superfamilies of ion channels (voltage- and ligand-gated ion channels; VGICs and LGICs) and summarize existing UAA tools, their mode of action, potential, caveats, and technical considerations to their use in illuminating ion channel structure and function.



中文翻译:

使用光敏氨基酸探测离子通道的结构和功能

在生物物理学和神经科学领域,用光远程控制和监视离子通道操作的方法正在迅速扩展。最近的发展通过利用通过遗传密码扩展技术并入的光敏非天然氨基酸(UAA)将光敏性直接引入蛋白质中。UAA的引入可实现独特的分子水平控制,并且与最大的时空分辨率和较差的光侵入性结合使用时,可以直接操纵和询问离子通道功能。在这里,我们回顾了光敏UAA在离子通道的两个超家族(电压和配体门控离子通道; VGIC和LGIC)中的各种应用,并总结了现有的UAA工具,其作用方式,电势,警告,

更新日期:2018-04-10
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