The fabrication, characterization and analytical performance were investigated for a phenol biosensor based on the covalent bonding of tyrosinase (TYR) onto a graphene oxide (GO)-modified glassy carbon electrode (GCE) via glutaraldehyde (GA). The surface morphology of the modified electrode was studied by atomic force microscope (AFM) and field-emission scanning electron microscopy (FE-SEM). The fabricated TYR/GA/GO/GCE biosensor showed very good stability, reproducibility, sensitivity and practical usage. The catechol biosensor exhibited a wide sensing linear range from 5 × 10⁻⁸ M to 5 × 10⁻⁵ M, a lower detection limit of 3 × 10⁻⁸ M, a current maximum (I_max) of 65.8 μA and an apparent Michaelis constant (K_m^app) of 169.9 μM.

基于酪氨酸酶（TYR）通过戊二醛（GA）共价键合到氧化石墨烯（GO）修饰的玻碳电极（GCE）上的酚类生物传感器的制备，表征和分析性能。通过原子力显微镜（AFM）和场发射扫描电子显微镜（FE-SEM）研究了修饰电极的表面形貌。所制造的TYR / GA / GO / GCE生物传感器显示出非常好的稳定性，可重复性，灵敏度和实际使用性。儿茶酚生物传感器的线性范围从5×10 ^-8  M到5×10 ^-5  M，检测下限为3×10 ^-8  M，电流最大值（I _max）为65.8μA，表观米氏（Michaelis）常数（K _m^app）为169.9μM。