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JOINTLESS Maintains Inflorescence Meristem Identity in Tomato Plant Cell Physiol. (IF 4.9) Pub Date : 2024-04-17 Samuel Huerga-Fernández, Nathalie Detry, Beata Orman-Ligeza, Frédéric Bouché, Marc Hanikenne, Claire Périlleux
JOINTLESS (J) was isolated in tomato (Solanum lycopersicum) from mutants lacking a flower pedicel abscission zone (AZ), and encodes a MADS-box protein of the SVP/AGL24 sub-family. The loss of J function also causes the return to leaf initiation in the inflorescences, indicating a pivotal role in inflorescence meristem identity. Here, we compared j mutants in different accessions that exhibit either
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Response and Adaptive Mechanism of Flavonoids in Pigmented Potatoes to Different Altitudes Plant Cell Physiol. (IF 4.9) Pub Date : 2024-04-16 Xiaojie Wu, Jiping Xiao
Altitude is an important ecological factor affecting plant physiology and ecology, material metabolism and gene expression. Tuber color changes were observed in purple and red potatoes growing at four different elevations ranging from 1800±50 to 3300±50 meters in the Tiger Leaping Gorge area of Yunnan Province. The results showed that the TPC, TFC, TAC and biological yield of anthocyanin increased
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The role of FtsH complexes in the response to abiotic stress in cyanobacteria Plant Cell Physiol. (IF 4.9) Pub Date : 2024-04-15 Vendula Krynická, Josef Komenda
FtsH proteases (FtsHs) belong to intramembrane ATP-dependent metalloproteases which are widely distributed in eubacteria, mitochondria, and chloroplasts. The best studied role of FtsH in Escherichia coli includes quality control of membrane proteins, regulation of response to heat shock, superoxide stress and viral infection, and control of lipopolysaccharide biosynthesis. While heterotrophic bacteria
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Functions and Mechanisms of Brassinosteroids in Regulating Crop Agronomic Traits Plant Cell Physiol. (IF 4.9) Pub Date : 2024-04-15 Xu Chen, Xiaotong Hu, Jianjun Jiang, Xuelu Wang
Brassinosteroids (BRs) perform crucial functions controlling plant growth and developmental processes, encompassing many agronomic traits in crops. Studies of BR-related genes involved in agronomic traits have suggested that BRs could serve as a potential target for crop breeding. Given the pleiotropic effect of BRs, a systematic understanding of their functions and molecular mechanisms is conducive
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Analytical Methods for Brassinosteroid Analysis: Recent Advances and Applications Plant Cell Physiol. (IF 4.9) Pub Date : 2024-04-15 Jana Oklestkova, Miroslav Kvasnica, Miroslav Strnad
Brassinosteroids (BRs) are plant steroidal hormones that play crucial roles in plant growth and development. Accurate quantification of BRs in plant tissues is essential for understanding their biological functions. This study presents a comprehensive overview of the latest methods used for the quantification of BRs in plants. We discuss the principles, advantages, and limitations of various analytical
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The Verticillium dahliae effector VdPHB1 promotes pathogenicity in cotton and interacts with the immune protein GhMC4 Plant Cell Physiol. (IF 4.9) Pub Date : 2024-04-14 Qingwei Song, Song Han, Shi Hu, Yiyang Xu, Kaijing Zuo
Verticillium dahliae is a kind of pathogenic fungus that brings about wilt disease and great losses in cotton. The molecular mechanism of the effectors in V. dahliae regulating cotton immunity remains largely unknown. Here we identified an effector of V. dahliae, VdPHB1, whose gene expression is highly induced by infection. VdPHB1 protein is localized in the intercellular space of cotton plants. Knockout
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The Arabidopsis Mitochondrial Nucleoid–Associated Protein WHIRLY2 Is Required for a Proper Response to Salt Stress Plant Cell Physiol. (IF 4.9) Pub Date : 2024-04-09 Yuri L Negroni, Irene Doro, Alberto Tamborrino, Irene Luzzi, Stefania Fortunato, Götz Hensel, Solmaz Khosravi, Laura Maretto, Piergiorgio Stevanato, Fiorella Lo Schiavo, Maria Concetta de Pinto, Karin Krupinska, Michela Zottini
In the last years, plant organelles have emerged as central coordinators of responses to internal and external stimuli, which can induce stress. Mitochondria play a fundamental role as stress sensors being part of a complex communication network between the organelles and the nucleus. Among the different environmental stresses, salt stress poses a significant challenge and requires efficient signaling
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Spatiotemporal Study of Galactolipid Biosynthesis in Duckweed with Mass Spectrometry Imaging and in vivo Isotope Labeling Plant Cell Physiol. (IF 4.9) Pub Date : 2024-04-09 Vy T Tat, Young Jin Lee
Isotope labeling coupled with mass spectrometry imaging (MSI) presents a potent strategy for elucidating the dynamics of metabolism in cellular resolution, yet its application to plant systems is scarce. It has the potential to reveal the spatiotemporal dynamics in lipid biosynthesis during plant development. In this study, we explore its application to galactolipid biosynthesis of an aquatic plant
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APX2 Is an Ascorbate Peroxidase–Related Protein that Regulates the Levels of Plastocyanin in Chlamydomonas Plant Cell Physiol. (IF 4.9) Pub Date : 2024-04-09 Anna Caccamo, Félix Vega de Luna, Agnieszka E Misztak, Sébastien Pyr dit Ruys, Didier Vertommen, Pierre Cardol, Joris Messens, Claire Remacle
The function of ascorbate peroxidase–related (APX-R) proteins, present in all green photosynthetic eukaryotes, remains unclear. This study focuses on APX-R from Chlamydomonas reinhardtii, namely, ascorbate peroxidase 2 (APX2). We showed that apx2 mutants exhibited a faster oxidation of the photosystem I primary electron donor, P700, upon sudden light increase and a slower re-reduction rate compared
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Redox regulation by priming agents towards a sustainable agriculture Plant Cell Physiol. (IF 4.9) Pub Date : 2024-04-09 Durgesh Kumar Tripathi, Javaid Akhtar Bhat, Chrystalla Antoniou, Nidhi Kandhol, Vijay Pratap Singh, Alisdair R Fernie, Vasileios Fotopoulos
Plant are sessile organisms that are often subjected to a multitude of environmental stresses, with the occurrence of these events being further intensified by global climate change. Crop species therefore require specific adaptations to tolerate climatic variability for sustainable food production. Plant stress results in excess accumulation of reactive oxygen species (ROS) leading to oxidative stress
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Shaping brassinosteroid signaling through scaffold proteins Plant Cell Physiol. (IF 4.9) Pub Date : 2024-04-09 Boyu Guo, Eun-Ji Kim, Yuxian Zhu, Kun Wang, Eugenia Russinova
Cellular responses to internal and external stimuli are orchestrated by intricate intracellular signaling pathways. To ensure an efficient and specific information flow, cells employ scaffold proteins as critical signaling organizers. With the ability to bind multiple signaling molecules, scaffold proteins can sequester signaling components within specific subcellular domains or modulate the efficiency
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The LysM receptor-like kinase SlLYK10 controls lipochitooligosaccharide signaling in inner cell layers of tomato roots Plant Cell Physiol. (IF 4.9) Pub Date : 2024-04-06 Yi Ding, Tongming Wang, Virginie Gasciolli, Guilhem Reyt, Céline Remblière, Fabien Marcel, Tracy François, Abdelhafid Bendahmane, Guanghua He, Jean Jacques Bono, Benoit Lefebvre
Establishment of arbuscular mycorrhiza (AM) relies on a plant signaling pathway that can be activated by fungal chitinic signals such as short chain chitooligosaccharides (CO) and lipo-chitooligosaccharides (LCOs). The tomato LysM receptor-like kinase (LysM RLK) SlLYK10 has high affinity for LCOs and is involved in root colonization by arbuscular mycorrhizal fungi (AMF), however its role in LCO responses
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Recent Advances in the Crosstalk between Brassinosteroids and Environmental Stimuli Plant Cell Physiol. (IF 4.9) Pub Date : 2024-04-05 Yuqing Zhao, Qing Han, Dawei Zhang
Due to their sessile lifestyle, plants need to optimize their growth in order to adapt to ever-changing environments. Plants receive stimuli from the environment and convert them into cellular responses. Brassinosteroids (BRs), as growth-promoting steroid hormones, play a significant role in the tradeoff between growth and environmental responses. Here, we provide a comprehensive summary for understanding
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CsHSFA1d Promotes Drought Stress Tolerance by Increasing the Content of Raffinose Family Oligosaccharides and Scavenging Accumulated Reactive Oxygen Species in Cucumber Plant Cell Physiol. (IF 4.9) Pub Date : 2024-04-02 Danhui Dong, Chuandong Qi, Jialong Zhang, Qilin Deng, Pingxin Xia, Ping Li, Congyang Jia, Bing Zhao, Na Zhang, Yang-Dong Guo
Drought is the most severe form of stress experienced by plants worldwide. Cucumber is a vegetable crop that requires a large amount of water throughout the growth period. In our previous study, we identified that overexpression of CsHSFA1d could improve cold tolerance and the content of endogenous jasmonic acid in cucumber seedlings. To explore the functional diversities of CsHSFA1d, we treat the
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Long-term consequences of PTI activation and its manipulation by root-associated microbiota Plant Cell Physiol. (IF 4.9) Pub Date : 2024-03-29 Ryohei Thomas Nakano, Tomohisa Shimasaki
In nature, plants are constantly colonized by a massive diversity of microbes engaged in mutualistic, pathogenic, or commensal relationships with the host. Molecular patterns present in these microbes activate pattern-triggered immunity (PTI), which detects microbes in the apoplast or at the tissue surface. Whether and how PTI distinguishes among soil-borne pathogens, opportunistic pathogens, and commensal
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Dual regulation of cytochrome P450 gene expression by two distinct small RNAs, a novel tasiRNA and miRNA, in Marchantia polymorpha Plant Cell Physiol. (IF 4.9) Pub Date : 2024-03-28 Yu-Ling Hung, Syuan-Fei Hong, Wei-Lun Wei, Shiuan Cheng, Jia-Zhen Yu, Veny Tjita, Qian-Yuan Yong, Ryuichi Nishihama, Takayuki Kohchi, John Bowman, Yuan-Chi Chien, Yen-Hsin Chiu, Ho-Chun Yang, Mei-Yeh Jade Lu, Zhao-Jun Pan, Chun-Neng Wang, Shih-Shun Lin
The miR390-derived TAS3 trans-acting short-interfering RNAs (tasiRNAs) module represents a conserved RNA silencing pathway in the plant kingdom; however, its characterization in the bryophyte Marchantia polymorpha is limited. This study elucidated that MpDCL4 processes MpTAS3 double-stranded RNA (dsRNA) to generate tasiRNAs, primarily from the 5ʹ- and 3ʹ-ends of dsRNA. Notably, we discovered a novel
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Biochemical characterization of rice xylan biosynthetic enzymes in determining xylan chain elongation and substitutions Plant Cell Physiol. (IF 4.9) Pub Date : 2024-03-19 Ruiqin Zhong, Dennis R Phillips, Kevin D Clark, Earle R Adams, Chanhui Lee, Zheng-Hua Ye
Grass xylan consists of a linear chain of β-1,4-linked xylosyl residues that often form domains substituted only with either arabinofuranose (Araf) or (methyl)glucuronic acid [(Me)GlcA] residues and it lacks the unique reducing end tetrasaccharide sequence found in dicot xylan. The mechanism of how grass xylan backbone elongation is initiated and how its distinctive substitution pattern is determined
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Bilirubin Distribution in Plants at the Subcellular and Tissue Levels Plant Cell Physiol. (IF 4.9) Pub Date : 2024-03-11 Kazuya Ishikawa, Yutaka Kodama
In heterotrophs, heme degradation produces bilirubin, a tetrapyrrole compound that has antioxidant activity. In plants, heme is degraded in plastids and is believed to be converted to phytochromobilin rather than bilirubin. Recently, we used the bilirubin-inducible fluorescent protein UnaG to reveal that plants produce bilirubin via a non-enzymatic reaction with NADPH. In the present study, we used
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Pleiotropic Effects of miR5504 Underlying Plant Height, Grain Yield and Quality in Rice Plant Cell Physiol. (IF 4.9) Pub Date : 2024-03-06 Huihui Wang, Xin Wang, Yangyang Li, Ying Cui, Xin Yan, Jiadong Gao, Jiexiu Ouyang, Shaobo Li
MicroRNAs (miRNAs) are known to play critical roles in regulating rice agronomic traits through mRNA cleavage or translational repression. Our previous study indicated that miR5504 regulates plant height by affecting cell proliferation and expansion. Here, the two independent homozygous mir5504 mutants (CR1 and CR2) and overexpression lines (OE1 and OE2) were further used to investigate the functions
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Light wavelength as a contributory factor of environmental fitness in the cyanobacterial circadian clock Plant Cell Physiol. (IF 4.9) Pub Date : 2024-03-05 Naohiro Kawamoto, Shuji Nakanishi, Ginga Shimakawa
The circadian clock is an essential system that drives the 24-hour expression rhythms for adaptation to day-night cycles. The molecular mechanism of the circadian clock has been extensively studied in cyanobacteria harboring the KaiC-based timing system. Nevertheless, our understanding of the physiological significance of the cyanobacterial circadian clock is still limited. In this study, we cultured
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High myristic acid in glycerolipids enhances the repair of photodamaged photosystem II under strong light Plant Cell Physiol. (IF 4.9) Pub Date : 2024-03-05 Kazuki Kurima, Haruhiko Jimbo, Takashi Fujihara, Masakazu Saito, Toshiki Ishikawa, Hajime Wada
Cyanobacteria inhabit areas with a broad range of light, temperature, and nutrient conditions. The robustness of cyanobacterial cells, which can survive under different conditions, may depend on the resilience of photosynthetic activity. Cyanothece sp. PCC 8801 (Cyanothece), a freshwater cyanobacterium isolated from a Taiwanese rice field, had a higher repair activity of photodamaged photosystem II
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SULTR2;1 Adjusts the Bolting Timing by Transporting Sulfate from Rosette Leaves to the Primary Stem Plant Cell Physiol. (IF 4.9) Pub Date : 2024-03-01 Khamsalath Soudthedlath, Toshiki Nakamura, Tsukasa Ushiwatari, Jutarou Fukazawa, Keishi Osakabe, Yuriko Osakabe, Akiko Maruyama-Nakashita
Sulfur (S) is an essential macronutrient for plant growth and metabolism. SULTR2;1 is a low-affinity sulfate transporter facilitating the long-distance transport of sulfate in Arabidopsis. The physiological function of SULTR2;1 in the plant life cycle still needs to be determined. Therefore, we analyzed the sulfate transport, S-containing metabolites accumulation, and plant growth using Arabidopsis
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Recent advances in understanding the regulatory mechanism of plasma membrane H+-ATPase through the brassinosteroid signaling pathway Plant Cell Physiol. (IF 4.9) Pub Date : 2024-02-19 Zhaoheng Lin, Pan Zhu, Liyang Gao, Xuanyi Chen, Meijing Li, Yuhe Wang, Junxian He, Ying Miao, Rui Miao
The polyhydroxylated steroid phytohormone brassinosteroids (BRs) control many aspects of plant growth, development and responses to environmental changes. Plasma membrane (PM) H+-ATPase, the well-known PM proton pump, is a central regulator in plant physiology, which mediates not only plant growth and development, but also adaptation to stresses. Recent studies highlight that PM H+-ATPase is at least
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Involvement of Peptidoglycan Receptor Proteins in Mediating the Growth-Promoting Effects of Bacillus pumilus TUAT1 in Arabidopsis thaliana. Plant Cell Physiol. (IF 4.9) Pub Date : 2024-02-19 Md Monirul Islam, Shin-ichiro Agake, Takehiro Ito, Safiullah Habibi, Michiko Yasuda, Tetsuya Yamasda, Gary Stacey, Naoko Ohkama-Ohtsu
Bacillus pumilus TUAT1 acts as plant growth-promoting rhizobacteria (PGPR) for various plants like rice and Arabidopsis. Under stress conditions, B. pumilus TUAT1 forms spores with a thick peptidoglycan (PGN) cell wall. Previous research showed that spores were significantly more effective than vegetative cells in enhancing plant growth. In Arabidopsis, the lysin-motif proteins LYM1, LYM3, and CERK1
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Divergent protein redox dynamics and their relationship with electron transport efficiency during photosynthesis induction Plant Cell Physiol. (IF 4.9) Pub Date : 2024-02-02 Keisuke Yoshida, Toru Hisabori
Various chloroplast proteins are activated/deactivated during the light/dark cycle via the redox regulation system. Although the photosynthetic electron transport chain provides reducing power to redox-sensitive proteins via the ferredoxin (Fd)/thioredoxin (Trx) pathway for their enzymatic activity control, how the redox states of individual proteins are linked to electron transport efficiency remains
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Restoration of the Functional nif Gene Cluster by Complex Recombination Events during Heterocyst Development in the Nitrogen-Fixing Cyanobacterium Calothrix sp. NIES-4101 Plant Cell Physiol. (IF 4.9) Pub Date : 2024-02-01 Kazuma Uesaka, Mari Banba, Sotaro Chiba, Yuichi Fujita
In the genome of the heterocystous cyanobacterium Calothrix sp. NIES-4101 (NIES-4101), the four genes essential for nitrogen fixation (nifB, nifH, nifD, and nifK) are highly fragmented into 13 parts in a 350-kb chromosomal region, and four of these parts are encoded in the reverse strand. Such a complex fragmentation feature makes it difficult to restore the intact nifBHDK genes by the excision mechanism
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Trans-species mobility of RNA interference between plants and associated organisms Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-30 Ya-Chi Nien, Allison Vanek, Michael J Axtell
Trans-species RNA interference occurs naturally when small RNAs (sRNA) silence genes in species different from their origin. This phenomenon has been observed between plants and various organisms including fungi, animals, and other plant species. Understanding the mechanisms used in natural cases of trans-species RNAi, such as sRNA processing and movement, will enable more effective development of
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DNA- and selectable-marker-free genome-editing system using zygotes from recalcitrant maize inbred B73 Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-30 Hajime Yamada, Norio Kato, Masako Ichikawa, Keiko Mannen, Takatoshi Kiba, Yuriko Osakabe, Hitoshi Sakakibara, Minami Matsui, Takashi Okamoto
Genome-editing tools such as the clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9) system have become essential tools for increasing the efficiency and accuracy of plant breeding. Using such genome-editing tools on maize, one of the most important cereal crops of the world, will greatly benefit the agriculture and the mankind. Conventional genome-editing methods typically
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Characterisation of Unique Eukaryotic Sphingolipids with Temperature-Dependent Δ8-Unsaturation from the Picoalga Ostreococcus Tauri Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-22 Toshiki Ishikawa, Frédéric Domergue, Alberto Amato, Florence Corellou
Sphingolipids are ubiquitous components of eukaryotic cell membranes and are found in some prokaryotic organisms and viruses. They are composed of a sphingoid backbone that may be acylated and glycosylated. Assembly of various sphingoid base, fatty-acyl and glycosyl moieties results in highly diverse structures. The functional significance of variations in sphingolipid chemical diversity and abundance
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BIL9 Promotes Both Plant Growth via BR Signaling and Drought Stress Resistance by Binding with the Transcription Factor HDG11 Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-19 Surina Surina, Ayumi Yamagami, Tomoko Miyaji, Zhana Chagan, Kiwi Mi Chung, Nobutaka Mitsuda, Kaisei Nishida, Ryo Tachibana, Zhangliang Zhu, Takuya Miyakawa, Kazuo Shinozaki, Masaaki Sakuta, Tadao Asami, Takeshi Nakano
Drought stress is a major threat leading to global plant and crop losses in the context of the climate change crisis. Brassinosteroids (BRs) are plant steroid hormones, and the BR signaling mechanism in plant development has been well elucidated. Nevertheless, the specific mechanisms of BR signaling in drought stress are still unclear. Here, we identify a novel Arabidopsis gene, BRZ INSENSITIVE LONG
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SOG1 and BRCA1 interdependently regulate RAD54 expression for repairing salinity-induced DNA double-strand breaks in Arabidopsis Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-19 Kalyan Mahapatra, Sujit Roy
As sessile organisms, land plants experience various forms of environmental stresses throughout their life span. Therefore, plants have developed extensive and complicated defence mechanisms, including a robust DNA damage response (DDR) and DNA repair systems for maintaining genome integrity. In Arabidopsis, the NAC domain family transcription factor SUPPRESSOR OF GAMMA RESPONSE1 (SOG1) plays an important
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Transcriptome analysis of rice root tips reveals auxin, gibberellin, and ethylene signalling underlying nutritropism Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-16 Kiyoshi Yamazaki, Yoshihiro Ohmori, Hirokazu Takahashi, Atsushi Toyoda, Yutaka Sato, Mikio Nakazono, Toru Fujiwara
Nutritropism is a positive tropism towards nutrients in plant roots. An NH4+ gradient is a nutritropic stimulus in rice (Oryza sativa L.). When rice roots are exposed to an NH4+ gradient generated around nutrient sources, root tips bend towards and coil around the sources. The molecular mechanisms are largely unknown. Here, we analysed the transcriptomes of the inside and outside of bending root tips
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Progesterone Metabolism In Digitalis And Other Plants - 60 Years Research And Recent Results Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-16 J Klein
Abstarct 5β-Cardenolides are pharmaceutically important metabolites of the specialized metabolism of Digitalis lanata. They were used over decades to treat cardiac insufficiency and supraventricular tachycardia. Since the 1960s, plant scientists have known that progesterone is an essential precursor of cardenolide formation. Therefore, plant progesterone biosynthesis was mainly analyzed in species
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Mutation of AtPME2, a pH-Dependent Pectin Methylesterase, Affects Cell Wall Structure and Hypocotyl Elongation Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-08 Ludivine Hocq, Olivier Habrylo, Fabien Sénéchal, Aline Voxeur, Corinne Pau-Roblot, Josip Safran, Françoise Fournet, Solène Bassard, Virginie Battu, Hervé Demailly, José C Tovar, Serge Pilard, Paulo Marcelo, Brett J Savary, Davide Mercadante, Maria Fransiska Njo, Tom Beeckman, Arezki Boudaoud, Laurent Gutierrez, Jérôme Pelloux, Valérie Lefebvre
Pectin methylesterases (PMEs) modify homogalacturonan’s chemistry and play a key role in regulating primary cell wall mechanical properties. Here, we report on Arabidopsis AtPME2, which we found to be highly expressed during lateral root emergence and dark-grown hypocotyl elongation. We showed that dark-grown hypocotyl elongation was reduced in knock-out mutant lines as compared to the control. The
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Thermospermine is an evolutionarily ancestral phytohormone required for organ development and stress responses in Marchantia polymorpha Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-05 Takuya Furumoto, Shohei Yamaoka, Takayuki Kohchi, Hiroyasu Motose, Taku Takahashi
Thermospermine suppresses auxin-inducible xylem differentiation, whereas its structural isomer, spermine, is involved in stress responses in angiosperms. The thermospermine synthase, ACAULIS5 (ACL5), is conserved from algae to land plants, but its physiological functions remain elusive in non-vascular plants. Here, we focused on MpACL5, a gene in the liverwort Marchantia polymorpha, that rescued the
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Comparative Analysis of Shikonin and Alkannin Acyltransferases Reveals Their Functional Conservation in Boraginaceae Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-05 Haruka Oshikiri, Hao Li, Misaki Manabe, Hirobumi Yamamoto, Kazufumi Yazaki, Kojiro Takanashi
Shikonin and its enantiomer, alkannin, are bioactive naphthoquinones produced in several plants of the family Boraginaceae. The structures of these acylated derivatives, which have various short-chain acyl moieties, differ among plant species. The acylation of shikonin and alkannin in Lithospermum erythrorhizon was previously reported to be catalyzed by two enantioselective BAHD acyltransferases, shikonin
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Transcription of the antisense long non-coding RNA, SUPPRESSOR OF FEMINIZATION, represses expression of the female-promoting gene FEMALE GAMETOPHYTE MYB in the liverwort Marchantia polymorpha Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-04 Tomoaki Kajiwara, Motoki Miyazaki, Shohei Yamaoka, Yoshihiro Yoshitake, Yukiko Yasui, Ryuichi Nishihama, Takayuki Kohchi
Sexual differentiation is a fundamental process in the life cycles of land plants, ensuring successful sexual reproduction and thereby contributing to species diversity and survival. In the dioicous liverwort Marchantia polymorpha, this process is governed by an autosomal sex-differentiation locus comprising FGMYB, a female-promoting gene, and SUF, an antisense strand-encoded long non-coding RNA (lncRNA)
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The Arabidopsis katamari2 Mutant Exhibits a Hypersensitive Seedling Arrest Response at the Phase Transition from Heterotrophic to Autotrophic Growth Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-04 Chika Hosokawa, Hiroki Yagi, Shoji Segami, Atsushi J Nagano, Yasuko Koumoto, Kentaro Tamura, Yoshito Oka, Tomonao Matsushita, Tomoo Shimada
Young seedlings use nutrients stored in the seeds to grow and acquire photosynthetic potential. This process, called seedling establishment, involves a developmental phase transition from heterotrophic to autotrophic growth. Some membrane-trafficking mutants of Arabidopsis (Arabidopsis thaliana), such as the katamari2 (kam2) mutant, exhibit growth arrest during seedling development, with a portion
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Broad Chain Length Specificity of the Alkane-Forming Enzymes NoCER1A and NoCER3A/B in Nymphaea odorata Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-04 Hisae Kojima, Kanta Yamamoto, Takamasa Suzuki, Yuri Hayakawa, Tomoko Niwa, Kenro Tokuhiro, Satoshi Katahira, Tetsuya Higashiyama, Sumie Ishiguro
Many terrestrial plants produce large quantities of alkanes for use in epicuticular wax and the pollen coat. However, their carbon chains must be long to be useful as fuel or as a petrochemical feedstock. Here, we focus on Nymphaea odorata, which produces relatively short alkanes in its anthers. We identified orthologs of the Arabidopsis alkane biosynthesis genes AtCER1 and AtCER3 in N. odorata and
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The Dicer-like Protein 4 and RNA-dependent RNA Polymerase 6 are Involved in Tomato Torrado Virus Pathogenesis in Nicotiana benthamiana Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-04 Przemysław Wieczorek, József Burgyán, Aleksandra Obrępalska-Stęplowska
Tomato torrado virus (ToTV) is a type member of the Torradovirus genus in the Secoviridae family known to cause severe necrosis in susceptible tomato varieties. ToTV also infects other Solanaceae plants, including Nicotiana benthamiana, where it induces distinctive disease symptoms: plant growth drop with the emergence of spoon-like malformed systemic leaves. Virus-induced post-transcriptional gene
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The function of florigen in the vegetative-to-reproductive phase transition in and around the shoot apical meristem Plant Cell Physiol. (IF 4.9) Pub Date : 2024-01-03 Hiroyuki Tsuji, Moeko Sato
Plants undergo a series of developmental phases throughout their life cycle, each characterized by specific processes. Three critical features distinguish these phases: the arrangement of primordia (phyllotaxis), the timing of their differentiation (plastochron), and the characteristics of the lateral organs and axillary meristems. Identifying the unique molecular features of each phase, determining
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Arabidopsis HECT and RING-type E3 Ligase Promote MAPKKK18 Degradation To Regulate Abscisic Acid Signalling Plant Cell Physiol. (IF 4.9) Pub Date : 2023-12-28 Małgorzata Tajdel-Zielińska, Maciej Janicki, Agnieszka Ludwików
Mitogen-activated protein kinase (MAPK) cascades are conserved signalling pathways that transduce extracellular signals into diverse cellular responses. Arabidopsis MAPKKK18 is a component of the MAP3K17/18-MKK3-MPK1/2/7/14 cascades, which play critical roles in ABA signalling, drought tolerance and senescence. A very important aspect of MAP kinase signalling is both its activation and its termination
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Detailing early shoot growth arrest in Kro-0 x BG-5 hybrids of Arabidopsis thaliana Plant Cell Physiol. (IF 4.9) Pub Date : 2023-12-28 Katelyn Sageman-Furnas, Gustavo T Duarte, Roosa A. E Laitinen
Shoot growth directly impacts plant productivity. Plants adjust their shoot growth in response to varying environments to maximize resource capture and stress resilience. While several factors controlling shoot growth are known, the complexity of the regulation and the input of the environment are not fully understood. We have investigated shoot growth repression induced by low ambient temperatures
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Glycyrrhizin Production in Licorice Hairy Roots Based on Metabolic Redirection of Triterpenoid Biosynthetic Pathway by Genome Editing Plant Cell Physiol. (IF 4.9) Pub Date : 2023-12-28 Naoki Chiyo, Hikaru Seki, Takuya Kanamoto, Hiroshi Ueda, Mareshige Kojoma, Toshiya Muranaka
Glycyrrhizin, a type of the triterpenoid saponin, is a major active ingredient contained in the roots of the medicinal plant licorice (Glycyrrhiza uralensis, G. glabra and G. inflata), and is used worldwide in diverse applications, such as herbal medicines and sweeteners. The growing demand for licorice threatens wild resources and therefore a sustainable method of supplying glycyrrhizin is required
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Temperature-Regulated Flowering Locus T Like gene Coordinates the Spike Initiation in Phalaenopsis orchid Plant Cell Physiol. (IF 4.9) Pub Date : 2023-12-28 Hsiang-Chia Lu, Chiao-Wen Huang, Tetsuro Mimura, Dewi Sukma, Ming-Tsair Chan
Phalaenopsis aphrodite can be induced to initiate spike growth and flowering by exposure to low ambient temperatures. However, the factors and mechanisms responsible for spike initiation in P. aphrodite remain largely unknown. In this study, we show that a repressor FLOWERING LOCUS T-like gene, FTL, can act as a negative regulator of spike initiation in P. aphrodite. The mRNA transcripts of PaFTL are
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DesC1 and DesC2, Δ9 Fatty Acid Desaturases of Filamentous Cyanobacteria: Essentiality and Complementarity Plant Cell Physiol. (IF 4.9) Pub Date : 2023-12-26 Devi B Effendi, Iwane Suzuki, Norio Murata, Koichiro Awai
DesC1 and DesC2, which are fatty acid desaturases found in cyanobacteria, are responsible for introducing a double bond at the Δ9 position of fatty-acyl chains, which are subsequently esterified to the sn-1 and sn-2 positions of the glycerol moiety, respectively. However, since the discovery of these two desaturases in the Antarctic cyanobacterium Nostoc sp. SO-36, no further research has been reported
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Arabidopsis HSFA9 acts as a regulator of heat response gene expression and the acquisition of thermotolerance and seed longevity Plant Cell Physiol. (IF 4.9) Pub Date : 2023-12-19 Xiaohua Wang, Yan Zhu, Ling Tang, Yuanyuan Wang, Runze Sun, Xin Deng
Heat shock transcription factors (HSFs) are crucial for regulating plant responses to heat and various stresses, as well as for maintaining normal cellular functions and plant development. HSFA9 and HSFA2 are two of the Arabidopsis class A HSFs and their expression are dramatically induced in response to heat shock (HS) stress among all 21 Arabidopsis HSFs. However, the detailed biological roles of
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The Molecular Frequency, Conservation and Role of Reactive Cysteines in Plant Lipid Metabolism Plant Cell Physiol. (IF 4.9) Pub Date : 2023-12-19 Ashley E Cannon, Patrick J Horn
Cysteines (Cys) are chemically reactive amino acids containing sulfur that play diverse roles in plant biology. Recent proteomics investigations in Arabidopsis thaliana have revealed the presence of thiol post-translational modifications (PTMs) on several Cys residues. These PTMs are presumed to impact protein structure and function, yet mechanistic data regarding the specific Cys susceptible to modification
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Genome editing of plant mitochondrial and chloroplast genomes Plant Cell Physiol. (IF 4.9) Pub Date : 2023-12-19 Shin-ichi Arimura, Issei Nakazato
Plastids (including chloroplasts) and mitochondria are remnants of endosymbiotic bacteria yet maintain their own genomes, which encode vital components for photosynthesis and respiration, respectively. Organellar genomes have distinctive features, such as being present as multicopies, being mostly inherited maternally, having characteristic genomic structures, and undergoing frequent homologous recombination
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Applications of Synthetic Pentatricopeptide Repeat Proteins Plant Cell Physiol. (IF 4.9) Pub Date : 2023-12-01 Farley Kwok van der Giezen, Suvi Honkanen, Catherine Colas des Francs-Small, Charles Bond, Ian Small
RNA binding proteins play integral roles in the regulation of essential processes in cells, and as such are attractive targets for engineering to manipulate gene expression at the RNA level. Expression of transcripts in chloroplasts and mitochondria is heavily regulated by pentatricopeptide repeat (PPR) proteins. The diverse roles of PPR proteins, and their naturally modular architecture, makes them
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Arabidopsis calmodulin-like proteins CML13 and CML14 interact with calmodulin-binding transcriptional activators and function in salinity stress response Plant Cell Physiol. (IF 4.9) Pub Date : 2023-11-29 Bryan Hau, Kyle Symonds, Howard Teresinski, Abby Janssen, Liam Duff, Milena Smith, Kirsten Benidickson, William Plaxton, Wayne A Snedden
Eukaryotic cells use calcium ions (Ca2+) as second messengers, particularly in response to abiotic and biotic stresses. These signals are detected by Ca2+ sensor proteins, such as calmodulin (CaM), which regulate downstream target proteins. Plants also possess many CaM-like proteins (CMLs), most of which remain unstudied. We recently demonstrated that Arabidopsis CML13 and CML14 interact with proteins
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Autophagy promotes cell death induced by hydrogen peroxide in Physcomitrium patens Plant Cell Physiol. (IF 4.9) Pub Date : 2023-11-29 Md Arif Sakil, Kyosuke Mukae, Junyu Bao, Abhishek Sadhu, Md. Shyduzzaman Roni, Yuko Inoue-Aono, Yuji Moriyasu
Autophagy-defective mutants (atg5 and atg7) of Physcomitrium patens exhibit strong desiccation tolerance. Here, we examined the effects of H2O2 on wild-type (WT) and autophagy-defective mutants of P. patens, considering that desiccation induces reactive oxygen species (ROS). We found that atg mutants can survive a 30 min treatment with 100 mM H2O2 whereas WT cannot, implying that autophagy promotes
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Inter-Organellar Effects of Defective ER-Localized Linolenic Acid Formation on Thylakoid Lipid Composition, Non-Photochemical Quenching of Chlorophyll Fluorescence and Xanthophyll Cycle Activity in the Arabidopsis fad3 Mutant Plant Cell Physiol. (IF 4.9) Pub Date : 2023-11-22 Monique Matzner, Larissa Launhardt, Olaf Barth, Klaus Humbeck, Reimund Goss, Ingo Heilmann
Monogalactosyldiacylglycerol (MGDG) is the main lipid constituent of thylakoids and a structural component of photosystems and photosynthesis-related proteo-lipid complexes in green tissues. Previously reported changes in MGDG abundance upon stress treatments are hypothesized to reflect mobilization of MGDG-based polyunsaturated lipid intermediates to maintain extraplastidial membrane integrity. While
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A Novel Soybean Diacylglycerol Acyltransferase 1b Variant with Three Amino Acid Substitutions Increases Seed Oil Content Plant Cell Physiol. (IF 4.9) Pub Date : 2023-11-20 Kayla S Flyckt, Keith Roesler, Kristin Haug Collet, Luciano Jaureguy, Russ Booth, Shawn R Thatcher, John D Everard, Kevin G Ripp, Zhan-Bin Liu, Bo Shen, Laura L Wayne
Improving soybean (Glycine max) seed composition by increasing the protein and oil components will add significant value to the crop and enhance environmental sustainability. Diacylglycerol acyltransferase (DGAT) catalyzes the final rate-limiting step in triacylglycerol (TAG) biosynthesis and has a major impact on seed oil accumulation. We previously identified a soybean DGAT1b variant with 14 amino
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Accumulation of Phosphorylated SnRK2-Substrate 1 Promotes Drought Escape in Arabidopsis Plant Cell Physiol. (IF 4.9) Pub Date : 2023-11-16 Sotaro Katagiri, Yoshiaki Kamiyama, Kota Yamashita, Sara Iizumi, Risa Suzuki, Yuki Aoi, Takahashi Fuminori, Hiroyuki Kasahara, Toshinori Kinoshita, Taishi Umezawa
Plants adopt optimal tolerance strategies depending on the intensity and duration of stress. Retaining water is a priority under short-term drought conditions, whereas maintaining growth and reproduction processes takes precedence over survival under conditions of prolonged drought. However, the mechanism underlying changes in the stress response depending on the degree of drought is unclear. Here
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Variety of Plant Oils: Species-Specific Lipid Biosynthesis Plant Cell Physiol. (IF 4.9) Pub Date : 2023-11-16 Alyssa C Clews, Brandon A Ulch, Monika Jesionowska, Jun Hong, Robert T Mullen, Yang Xu
Plant oils represent a large group of neutral lipids with important applications in food, feed and oleochemical industries. Most plants accumulate oils in the form of triacylglycerol within seeds and their surrounding tissues, which is comprised of three fatty acids attached to a glycerol backbone. Different plant species accumulate unique fatty acids in their oils, serving a range of applications
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Volatile Compounds Emitted by Plant Growth–Promoting Fungus Tolypocladium inflatum GT22 Alleviate Copper and Pathogen Stress Plant Cell Physiol. (IF 4.9) Pub Date : 2023-11-12 Chih-Yun Chiang, Ching-Han Chang, Tzu-Yun Tseng, Van-Anh Thi Nguyen, Pei-Yu Su, Tu-Trinh Thi Truong, Jing-Yu Chen, Chung-Chih Huang, Hao-Jen Huang
Previous studies on the intricate interactions between plants and microorganisms have revealed that fungal volatile compounds (VCs) can affect plant growth and development. However, the precise mechanisms underlying these actions remain to be delineated. In this study, we discovered that VCs from the soilborne fungus Tolypocladium inflatum GT22 enhance the growth of Arabidopsis. Remarkably, priming
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Anthriscus sylvestris Deoxypodophyllotoxin Synthase Involved in Podophyllotoxin Biosynthesis Plant Cell Physiol. (IF 4.9) Pub Date : 2023-11-10 Keisuke Kobayashi, Masaomi Yamamura, Bunzo Mikami, Akira Shiraishi, Masato Kumatani, Honoo Satake, Eiichiro Ono, Toshiaki Umezawa
Tetrahydrofuran ring formation from dibenzylbutyrolactone lignans is a key step in the biosynthesis of aryltetralin lignans including deoxypodophyllotoxin and podophyllotoxin. Previously, Fe (II)- and 2-oxoglutarate-dependent dioxygenase (2-ODD) from Podophyllum hexandrum (Himalayan mayapple, Berberidaceae) was found to catalyze cyclization of a dibenzylbutyrolactone lignan, yatein, to give deoxypodophyllotoxin
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A carbonic anhydrase, ZmCA4, contributes to photosynthetic efficiency, and modulates CO2 signaling gene expression by interacting with aquaporin ZmPIP2;6 in maize Plant Cell Physiol. (IF 4.9) Pub Date : 2023-11-10 Lian Zhou, Xiaoqin Xiang, Dongpu Ji, Qiulan Chen, Tengfei Ma, Jiuguang Wang, Chaoxian Liu
Carbonic anhydrase (CA) catalyzes the reversible CO2 hydration reaction that produces bicarbonate for phosphoenolpyruvate carboxylase (PEPC). This is the initial step for transmitting the CO2 signal in C4 photosynthesis. However, it remains unknown whether the maize (Zea mays L.) carbonic anhydrase gene, ZmCA4, plays a role in maize photosynthesis process. In our study, we found that ZmCA4 was relatively
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Wounding Triggers Wax Biosynthesis in Arabidopsis Leaves in an Abscisic Acid and Jasmonoyl-Isoleucine Dependent Manner Plant Cell Physiol. (IF 4.9) Pub Date : 2023-11-06 Milena Lewandowska, Krzysztof Zienkiewicz, Agnieszka Zienkiewicz, Amélie Kelly, Stefanie König, Kirstin Feussner, Ljerka Kunst, Ivo Feussner
Wounding caused by insects or abiotic factors such as wind and hail can cause severe stress for plants. Intrigued by the observation that wounding induces expression of genes involved in surface wax synthesis in a jasmonoyl-isoleucine (JA-Ile)-independent manner, the role of wax biosynthesis and respective genes upon wounding was investigated. Wax, a lipid-based barrier, protects plants both from environmental