Reproductive dysfunction associated with obesity is increasing among women of reproductive age, including infertility and increasing risk of miscarriage. In females, reproductive disorders are linked to declining quality of oocytes. Using a model of diet-induced obesity, we have investigated the possible effects of obesity on oocyte quality, including metabolism, lipid accumulation, ROS levels, meiosis and changes in spindle structure in Metaphase II. Our study showed that obesity induced by a high fat diet can impair oocyte meiosis, destroy spindle assembly, and promote oxidative stress and abnormal mitochondrial distribution. With the addition of resveratrol, the negative impact of diet-induced obesity on the quality of oocytes was alleviated to some extent. In addition, we found that obesity causes mouse oocytes to soften, and resveratrol can restore the zona pellucida of oocytes to the same state as the control group. In conclusion, resveratrol can reverse the adverse effects of obesity on oocytes, which is beneficial for subsequent embryonic development.

中文翻译：

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白藜芦醇逆转了饮食诱导的肥胖鼠模型对卵母细胞质量和透明带软化的不利影响†

肥胖相关的生殖功能障碍在育龄妇女中正在增加，包括不孕症和流产的风险增加。在女性中，生殖障碍与卵母细胞质量下降有关。使用饮食诱发的肥胖症模型，我们研究了肥胖症对卵母细胞质量的可能影响，包括代谢，脂质堆积，ROS水平，减数分裂和中期II纺锤体结构变化。我们的研究表明，高脂饮食引起的肥胖会损害卵母细胞减数分裂，破坏纺锤体组装，促进氧化应激和异常线粒体分布。随着白藜芦醇的添加，饮食诱导的肥胖对卵母细胞质量的负面影响有所减轻。此外，我们发现肥胖会导致小鼠卵母细胞软化，白藜芦醇可使卵母细胞透明带恢复至与对照组相同的状态。总之，白藜芦醇可以逆转肥胖对卵母细胞的不利影响，这对随后的胚胎发育是有益的。